Journal article
Extensive sequence variation of feline immunodeficiency virusenv genes in isolates from naturally infected cats
Archives of Virology, Vol.133(1-2), pp.51-62
1993
Abstract
In an investigation of the evolution of feline immunodeficiency virus (FIV) in vivo, sequential isolates from a persistently infected cat were examined by direct sequencing following amplification of selected subgenomic regions by polymerase chain reaction (PCR). Three isolates, T 90, T 91, and T 92, obtained over a three-year period revealed no changes to regions known to be conserved within gag and pol genes. Additionally, no change occurred within gag and pol in an isolate recovered from a second cat which was experimentally infected with T 90. Changes were detected within an N-terminal region of the envelope glycoprotein gp 120 (env). These consisted of point mutations, some of which would result in amino acid substitutions and the predicted amino acid changes tended to cluster within variable domains. Inoculation of T 90 into a second cat resulted in a different pattern of mutations than that observed for the three isolates from the first cat. In all cases, virus isolates derived from the same cat were much more highly related to each other (extent of env variation was 0.5-1.5%) than to isolates from other cats (10-12%env variation). The rate of change of FIV was estimated to be 3.4×10-3 nucleotide substitutions per site per year for the env gene and less than 10-4 nucleotide substitutions per site per year for the gag and pol genes, values concordant with that found for human immunodeficiency virus 1. Both nucleotide and amino acid changes in the gp 120 region were found to be directional, suggesting that selective pressures influence FIV envelope gene sequences.
Details
- Title
- Extensive sequence variation of feline immunodeficiency virusenv genes in isolates from naturally infected cats
- Authors/Creators
- W.K. Greene (Author/Creator)J. Meers (Author/Creator)G. del Fierro (Author/Creator)P.R. Carnegie (Author/Creator)W.F. Robinson (Author/Creator)
- Publication Details
- Archives of Virology, Vol.133(1-2), pp.51-62
- Publisher
- Springer Verlag
- Identifiers
- 991005542997207891
- Copyright
- © 1993 Springer-Verlag
- Murdoch Affiliation
- School of Environmental Science; School of Veterinary Studies
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites
Metrics
47 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Citation topics
- 1 Clinical & Life Sciences
- 1.66 HIV
- 1.66.46 HIV Pathogenesis
- Web Of Science research areas
- Virology
- ESI research areas
- Microbiology