Faecal excretion of intestinal spirochaetes by urban dogs, and their pathogenicity in a chick model of intestinal spirochaetosis

N. Prapasarakul; K. Lugsomya; S. Disatian; T. Lekdumrongsak; W. Banlunara; P. Chetanachan; D.J. Hampson

doi:10.1016/j.rvsc.2011.01.015

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Faecal excretion of intestinal spirochaetes by urban dogs, and their pathogenicity in a chick model of intestinal spirochaetosis

Journal article

Peer reviewed

Faecal excretion of intestinal spirochaetes by urban dogs, and their pathogenicity in a chick model of intestinal spirochaetosis

N. Prapasarakul, K. Lugsomya, S. Disatian, T. Lekdumrongsak, W. Banlunara, P. Chetanachan and D.J. Hampson

Research in Veterinary Science, Vol.91(3), pp.e38-e43

2011

DOI: https://doi.org/10.1016/j.rvsc.2011.01.015

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Abstract

This study aimed to obtain information about the types of spirochaetes colonising urban dogs in Thailand, and to investigate their pathogenic potential in a day-old chick model of intestinal spirochaetosis. Spirochaetes were isolated from the faeces of six of 47 (12.8%) healthy dogs and 11 of 104 (10.6%) dogs with diarrhoea. Their biochemical properties and 16S ribosomal DNA sequences were analysed. Four isolates were identified as Brachyspira pilosicoli, three resembled "Brachyspira pulli", nine clustered with "Brachyspira canis" and one was similar to Brachyspira intermedia. Canine isolates of B. pilosicoli, "B. canis" and "B. pulli", and control strains of Brachyspira hyodysenteriae, B. pilosicoli and Brachyspira innocens colonised experimentally infected day-old chicks. The chicks did not develop diarrhoea, but were significantly lighter than the non-infected group and those infected with B. innocens after 21. days (P< 0.05). Using immunohistochemistry, spirochaetes were observed covering the surface epithelium and in the crypts of chicks in all three groups challenged with the canine isolates. Variable histopathological changes were seen, with the greatest inflammatory cell infiltration into the lamina propria occurring in the group infected with "B. pulli" Canine "B. canis", "B. pulli" and B. pilosicoli isolates may have pathogenic potential.

Details

Title: Faecal excretion of intestinal spirochaetes by urban dogs, and their pathogenicity in a chick model of intestinal spirochaetosis
Authors/Creators: N. Prapasarakul (Author/Creator) - Chulalongkorn University
K. Lugsomya (Author/Creator) - Chulalongkorn University
S. Disatian (Author/Creator) - Chulalongkorn University
T. Lekdumrongsak (Author/Creator) - Chulalongkorn University
W. Banlunara (Author/Creator) - Chulalongkorn University
P. Chetanachan (Author/Creator) - Department of Medical Science, National Institute of Medical Science, Bangkok 11000, Thailand
D.J. Hampson (Author/Creator) - Murdoch University
Publication Details: Research in Veterinary Science, Vol.91(3), pp.e38-e43
Publisher: W. B. Saunders Co., Ltd.
Identifiers: 991005542949807891
Copyright: © 2011 Elsevier Ltd
Murdoch Affiliation: School of Veterinary and Biomedical Sciences
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 1 Clinical & Life Sciences; 1.248 Sexually Transmitted Infections; 1.248.2445 Lawsonia Intracellularis
Web Of Science research areas: Veterinary Sciences
ESI research areas: Plant & Animal Science