Journal article
Ferguson plots based on absolute mobilities in polyacrylamide gel electrophoresis: Dependence of linearity of polymerization conditions and application to the determination of free mobility
Electrophoresis, Vol.9(7), pp.293-298
1988
Abstract
In contrast to Ferguson plots based on relative mobilities, Ferguson plots of proteins in polyacrylamide gel electrophoresis based on their absolute mobilities were found to be linear under unusual polymerization conditions which yield relatively wide gel fibers and a low total fiber length per unit weight, but not under previously and commonly used conditions. These linear Ferguson plots in gels of 1, 3 and 5% crosslinking intersect at a single gel concentration between 1 and 2 %T (M-point). It is postulated that the measure of free mobility of the proteins is the M-point, and not the intercept of their Ferguson plots with the mobility axis as assumed previously. This postulate abolishes the well-known paradoxical interpretation of the increase with %C of the linearly extrapolated intercept of the Ferguson plot with the log(mobility) axis (designated Yo) in terms of free mobility. The postulate is also compatible with the interpretation of the points of intersection of the Ferguson plots of oligomeric series of proteins at finite gel concentrations (designated μ-points) as their common free mobilities.
Details
- Title
- Ferguson plots based on absolute mobilities in polyacrylamide gel electrophoresis: Dependence of linearity of polymerization conditions and application to the determination of free mobility
- Authors/Creators
- M. Butterman (Author/Creator) - Health and Human Development (2HD) Research NetworkD. Tietz (Author/Creator) - Health and Human Development (2HD) Research NetworkL. Orbán (Author/Creator) - Health and Human Development (2HD) Research NetworkA. Chrambach (Author/Creator) - Health and Human Development (2HD) Research Network
- Publication Details
- Electrophoresis, Vol.9(7), pp.293-298
- Publisher
- John Wiley & Sons Ltd
- Identifiers
- 991005543411907891
- Copyright
- © 1988 VCH Verlagsgesellschaft mbH
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 2 Chemistry
- 2.166 Chromatography & Electrophoresis
- 2.166.730 Capillary Electrophoresis
- Web Of Science research areas
- Biochemical Research Methods
- Chemistry, Analytical
- ESI research areas
- Chemistry