Journal article
Fibroblast growth on micro- and nanopatterned surfaces prepared by a novel sol–gel phase separation method
Journal of Materials Science: Materials in Medicine, Vol.24(3), pp.783-792
2013
Abstract
Physical characteristics of the growth substrate including nano- and microstructure play crucial role in determining the behaviour of the cells in a given biological context. To test the effect of varying the supporting surface structure on cell growth we applied a novel sol–gel phase separation-based method to prepare micro- and nanopatterned surfaces with round surface structure features. Variation in the size of structural elements was achieved by solvent variation and adjustment of sol concentration. Growth characteristics and morphology of primary human dermal fibroblasts were found to be significantly modulated by the microstructure of the substrate. The increase in the size of the structural elements, lead to increased inhibition of cell growth, altered morphology (increased cytoplasmic volume), enlarged cell shape, decrease in the number of filopodia) and enhancement of cell senescence. These effects are likely mediated by the decreased contact between the cell membrane and the growth substrate. However, in the case of large surface structural elements other factors like changes in the 3D topology of the cell’s cytoplasm might also play a role.
Details
- Title
- Fibroblast growth on micro- and nanopatterned surfaces prepared by a novel sol–gel phase separation method
- Authors/Creators
- P. Reemann (Author/Creator)T. Kangur (Author/Creator)M. Pook (Author/Creator)M. Paalo (Author/Creator)L. Nurmis (Author/Creator)I. Kink (Author/Creator)O. Porosaar (Author/Creator)K. Kingo (Author/Creator)E. Vasar (Author/Creator)S. Kõks (Author/Creator)V. Jaks (Author/Creator)M. Järvekülg (Author/Creator)
- Publication Details
- Journal of Materials Science: Materials in Medicine, Vol.24(3), pp.783-792
- Publisher
- Springer US
- Identifiers
- 991005544802007891
- Copyright
- © 2012 Springer Science+Business Media New York
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites
Metrics
44 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.96 Cell Biology
- 1.96.224 Cell Mechanics
- Web Of Science research areas
- Engineering, Biomedical
- Materials Science, Biomaterials
- ESI research areas
- Materials Science