Journal article
First genetic characterisation of Giardia in human isolates from Jordan
Parasitology Research, Vol.115(10), pp.3723-3729
2016
Abstract
Little is known about the epidemiology of Giardia in Jordan and to date, no genotyping studies have been conducted on Giardia isolates from Jordanians. In the present study, a total of 49 microscopy-positive faecal samples from Jordanian patients suffering from giardiasis were analysed at two loci: the triose phosphate isomerase (tpi) gene and the glutamate dehydrogenase (gdh) gene. At the tpi locus, a total of 28 samples amplified and assemblage A was identified in 46.4 % (13/28) samples, while assemblage B was identified in 50 % (14/28) samples and a mixed assemblage A and B was identified in one sample (3.6 %) (Table 1). At the gdh locus 48 isolates amplified and of these assemblages A was identified in 43.7 % (21/48) of isolates and assemblage B in 56.3 % (27/48) of isolates. No mixed infections were detected at the gdh locus. Subtyping at the gdh locus identified sub-assemblage AII in 43.7 % (21/48) of isolates and sub-assemblages BIII and BIV in 25 % (12/48) and 31.2 % (15/48) of isolates, respectively, with more genetic diversity in AII isolates than BIII or BIV isolates. Novel sub-types within each sub-assemblage were identified suggesting unique endemicity and anthroponotic transmission of Giardia in Jordanian patients suffering from giardiasis. Further studies are required to better understand the epidemiology and transmission of Giardia in Jordan.
Details
- Title
- First genetic characterisation of Giardia in human isolates from Jordan
- Authors/Creators
- N. Hijjawi (Author/Creator) - Hashemite UniversityR. Yang (Author/Creator) - Murdoch UniversityR. Mukbel (Author/Creator) - Jordan University of Science and TechnologyY. Yassin (Author/Creator) - Hashemite UniversityT. Mharib (Author/Creator) - Hashemite UniversityU. Ryan (Author/Creator) - Murdoch University
- Publication Details
- Parasitology Research, Vol.115(10), pp.3723-3729
- Publisher
- Springer Verlag
- Identifiers
- 991005542699607891
- Copyright
- © 2016 Springer-Verlag Berlin Heidelberg
- Murdoch Affiliation
- School of Veterinary and Life Sciences
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 1 Clinical & Life Sciences
- 1.246 Diarrheal Diseases
- 1.246.985 Cryptosporidium
- Web Of Science research areas
- Parasitology
- ESI research areas
- Microbiology