Journal article
Follicle development, endocrine profiles and ovulation rate in adult Merino ewes: effects of early nutrition (pre- and post-natal) and supplementation with lupin grain
Reproduction, Vol.147(1), pp.101-110
2014
Abstract
In adult ewes, we tested whether ovarian function, including the response to short-term supplementation, was affected by the nutrition of their mothers during the pre-/post-natal period. A 2×2 factorial design was used with nutrition in early life (low or high) and a 6-day supplement (with or without) as factors. All ewes received three prostaglandin (PG) injections 7 days apart, and the supplement (lupin grain) was fed for 6 days from 2 days after the second until the third PG injection.We measured reproductive and metabolic hormones, studied follicle dynamics (ultrasonography), and evaluated granulosa cellnumbers, aromatase activity and oestradiol (E2) concentrations in follicular fluid in healthy follicles at days 3 and 7 of supplementation. Ovulation rate was increased by 25% by exposure to high pre-/post-natal nutrition (1.5 vs 1.2; P<0.05), in association with a small decrease in FSH concentrations (P=0.06) and a small increase in insulin concentrations (P=0.07). The number of healthy antral follicleswas not affected. Acute supplementation increased the number of granulosa cells (3.7±0.2 vs 3.0±0.2 million; P<0.05) in the largest follicle, and the circulating concentrations of E2 (4.6±0.3 vs 3.9±0.3 pmol/l; P<0.05) and glucose (3.4±0.03 vs 3.3±0.03 mmol/l; P<0.01). Both early life nutrition and acute supplementation appear to affect ovulation rate through changes in glucose-insulin homoeostasis that alter follicular responsiveness to FSH and therefore E2-FSH balance.
Details
- Title
- Follicle development, endocrine profiles and ovulation rate in adult Merino ewes: effects of early nutrition (pre- and post-natal) and supplementation with lupin grain
- Authors/Creators
- C. Vinoles (Author/Creator) - The University of Western AustraliaB.L. Paganoni (Author/Creator) - The University of Western AustraliaK.P. McNatty (Author/Creator) - The University of Western AustraliaD.A. Heath (Author/Creator) - The University of Western AustraliaA.N. Thompson (Author/Creator) - The University of Western AustraliaK.M.M. Glover (Author/Creator)J.T.B. Milton (Author/Creator)G.B. Martin (Author/Creator) - The University of Western Australia
- Publication Details
- Reproduction, Vol.147(1), pp.101-110
- Publisher
- Society for Reproduction and Fertility
- Identifiers
- 991005541309607891
- Copyright
- © 2013 Society for Reproduction and Fertility.
- Murdoch Affiliation
- School of Veterinary and Life Sciences
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites
Metrics
177 File views/ downloads
101 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.51 Dairy & Animal Sciences
- 3.51.115 Livestock Reproduction
- Web Of Science research areas
- Developmental Biology
- Reproductive Biology
- ESI research areas
- Molecular Biology & Genetics