Journal article
Fructose metabolism in wild-type, fructokinase-negative and revertant strains of Rhizobium leguminosarum
Microbiology, Vol.130(2), pp.231-237
1984
Abstract
Rhizobium leguminosarum accumulates fructose by an active process sensitive to azide, 2,4-dinitrophenol and carbonyl cyanide m-chlorophenylhydrazone. The fructose is not phosphorylated during transport. Sorbose and glucose interfere with fructose uptake. Inside the cell fructose is metabolized via fructose 6-phosphate; there is no evidence for an alternative metabolic route via sorbitol to glucose or via sorbitol 6-phosphate to fructose 6-phosphate. Tn5-induced mutants lacking fructokinase failed to grow on fructose, mannitol or sorbitol and grew slowly on sucrose; growth was normal on all other single carbon sources tested. Growth of these mutants on a range of carbon sources was retarded by added fructose. Revertants which had regained the capacity to utilize fructose all had an unstable fructokinase which could be partially stabilized by fructose.
Details
- Title
- Fructose metabolism in wild-type, fructokinase-negative and revertant strains of Rhizobium leguminosarum
- Authors/Creators
- A.R. Glenn (Author/Creator)R. Arwas (Author/Creator)I.A. McKay (Author/Creator)M.J. Dilworth (Author/Creator)
- Publication Details
- Microbiology, Vol.130(2), pp.231-237
- Publisher
- Society for General Microbiology
- Identifiers
- 991005541398907891
- Copyright
- © 1984 SGM
- Murdoch Affiliation
- School of Environmental and Life Sciences
- Language
- English
- Resource Type
- Journal article
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