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Published (Version of Record)CC BY-NC-ND V4.0, Open Access
Journal article
Fumarylacetoacetate hydrolase targeted by a Fusarium graminearum effector positively regulates wheat FHB resistance
Nature communications, Vol.16(1), 5582
2025
Abstract
Fusarium head blight (FHB), caused by Fusarium graminearum is a devastating disease that affects global wheat production. F. graminearum encodes many effector proteins; however, its virulence mechanisms are poorly understood. In this study, we identify a secretory effector candidate (FgEC10) that is essential for the virulence of F. graminearum. FgEC10 interacts strongly with wheat fumarylacetoacetate hydrolase (TaFAH) and accelerates its degradation via the 26S proteasome pathway. In addition, we show that TaFAH interacts with proteasome 26S subunit, non-ATPases 12 (TaPSMD12) and that FgEC10 enhances the interaction between TaFAH and TaPSMD12. RNA silencing or overexpression of TaFAH in wheat plants shows that TaFAH positively regulates wheat FHB resistance. Overexpression of TaFAH promotes the expression of genes associated with disease resistance and the heading period. Metabolomic analysis reveals that overexpression of TaFAH increases the levels of several amino acids in wheat, and exogenous application of some of these amino acids show an increase in F. graminearum resistance in the wheat spike and seedling. Collectively, our study reveals a pathogenic mechanism and provides a valuable gene resource for improving FHB resistance and promoting heading in wheat.
Details
- Title
- Fumarylacetoacetate hydrolase targeted by a Fusarium graminearum effector positively regulates wheat FHB resistance
- Authors/Creators
- Shengping Shang - Agricultural Genomics Institute at ShenzhenYuhan He - Agricultural Genomics Institute at ShenzhenRuihua Zhao - Agricultural Genomics Institute at ShenzhenHanqi Li - Agricultural Genomics Institute at ShenzhenYing Fang - Huazhong Agricultural UniversityQianyong Hu - Agricultural Genomics Institute at ShenzhenYujin Fan - Agricultural Genomics Institute at ShenzhenYiwei Wang - Agricultural Genomics Institute at ShenzhenXishi Zhou - Agricultural Genomics Institute at ShenzhenPenghao Wang - Murdoch UniversityXiaoping Xing - Henan Agricultural UniversityCui-Jun Zhang - Agricultural Genomics Institute at Shenzhen
- Publication Details
- Nature communications, Vol.16(1), 5582
- Publisher
- Nature Publishing Group UK
- Identifiers
- 991005787887507891
- Copyright
- © The Author(s) 2025
- Murdoch Affiliation
- Centre for Crop and Food Innovation; School of Medical, Molecular and Forensic Sciences
- Language
- English
- Resource Type
- Journal article
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