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Journal article
Functional analysis of polyphenol oxidase 1 gene in common wheat
Frontiers in plant science, Vol.14, 1171839
2023
Abstract
Polyphenol oxidase (PPO) activity is a major cause of the undesirable brown color of wheat-based products. Ppo1, a major gene for PPO activity, was cloned based on sequence homology in previous studies; however, its function and regulation mechanism remain unclear. In this study, the function and genetic regulation of Ppo1 were analyzed using RNA interference (RNAi) and Targeting Induced Local Lesions IN Genomes (TILLING) technology, and superior mutants were identified. Compared with the control, the level of Ppo1 transcript in RNAi transgenic lines was drastically decreased by 15.5%–60.9% during grain development, and PPO activity was significantly reduced by 12.9%–20.4%, confirming the role of Ppo1 in PPO activity. Thirty-two Ppo1 mutants were identified in the ethyl methanesulfonate (EMS)-mutagenized population, including eight missense mutations, 16 synonymous mutations, and eight intron mutations. The expression of Ppo1 was reduced significantly by 6.7%–37.1% and 10.1%–54.4% in mutants M092141 (G311S) and M091098 (G299R), respectively, in which PPO activity was decreased by 29.7% and 28.8%, respectively, indicating that mutation sites of two mutants have important effects on PPO1 function. Sequence and structure analysis revealed that the two sites were highly conserved among 74 plant species, where the frequency of glycine was 94.6% and 100%, respectively, and adjacent to the entrance of the hydrophobic pocket of the active site. The M092141 and M091098 mutants can be used as important germplasms to develop wheat cultivars with low grain PPO activity. This study provided important insights into the molecular mechanism of Ppo1 and the genetic improvement of wheat PPO activity.
Details
- Title
- Functional analysis of polyphenol oxidase 1 gene in common wheat
- Authors/Creators
- Shengnan Zhai - Ministry of Agriculture and Rural AffairsHang Liu - , , , , , ,Xianchun Xia - Institute of Crop SciencesHaosheng Li - Ministry of Agriculture and Rural AffairsXinyou Cao - Ministry of Agriculture and Rural AffairsZhonghu He - Institute of Crop SciencesWujun Ma - Murdoch University, Centre for Crop and Food InnovationCheng Liu - Ministry of Agriculture and Rural AffairsJianmin Song - Ministry of Agriculture and Rural AffairsAifeng Liu - Ministry of Agriculture and Rural AffairsJingjuan Zhang - Murdoch University, Centre for Crop and Food InnovationJianjun Liu - Ministry of Agriculture and Rural Affairs
- Publication Details
- Frontiers in plant science, Vol.14, 1171839
- Publisher
- Frontiers Media S.A
- Identifiers
- 991005599167307891
- Copyright
- © 2023 Zhai et al.
- Murdoch Affiliation
- Australia-China Joint Centre for Wheat Improvement; State Agricultural Biotechnology Centre
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.85 Food Science & Technology
- 3.85.99 Cereal Starch Properties
- Web Of Science research areas
- Plant Sciences
- ESI research areas
- Plant & Animal Science