Journal article
Further insight into the heparin-releasable and glycosylphosphatidylinositol-lipid—anchored forms of tissue factor pathway inhibitor
Clinical and Applied Thrombosis/Hemostasis, Vol.14(3), pp.267-278
2008
Abstract
The release of tissue factor pathway inhibitor (TFPI) from human umbilical vein endothelial cells (HUVECs) was investigated using heparin and phospholipase C. The experiment included incubating HUVECs with 0, 1, or 10 U/mL heparin diluted in Dulbecco Modified Eagle's Medium plus 5% fetal calf serum for 1 or 24 hours. A statistically significant increase in TFPI activity levels was seen at 1 hour, but not at 24 hours. A 20-fold increase in the release of TFPI after phospholipase C treatment of HUVECs was demonstrated, confirming that it is glycosylphosphatidylinositol-lipid (GPI) anchored. Sequential treatment of HUVECs with phospholipase C and heparin was performed, and a trend was observed where GPI-anchored TFPI levels were increased after 1 hour of pretreatment with heparin but were decreased after 24 hours. Serum is a requirement for the heparin-dependent release of TFPI from HUVECs. Heparin pretreatment of HUVECs may affect levels of GPI anchored TFPI in a time and dose-dependent manner.
Details
- Title
- Further insight into the heparin-releasable and glycosylphosphatidylinositol-lipid—anchored forms of tissue factor pathway inhibitor
- Authors/Creators
- P.E.R. Ellery (Author/Creator) - Curtin UniversityK. Hardy (Author/Creator) - Curtin UniversityR. Oostryck (Author/Creator) - Curtin UniversityM.J. Adams (Author/Creator) - Curtin University
- Publication Details
- Clinical and Applied Thrombosis/Hemostasis, Vol.14(3), pp.267-278
- Publisher
- SAGE Publications
- Identifiers
- 991005544934507891
- Copyright
- © 2008 Sage Publications
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.75 Blood Clotting
- 1.75.1434 Coagulation Pathways
- Web Of Science research areas
- Hematology
- Peripheral Vascular Disease
- ESI research areas
- Clinical Medicine