Journal article
Further investigations of the effects of anti-β2GP1 antibodies on collagen-induced platelet aggregation
Clinical and Applied Thrombosis/Hemostasis, Vol.24(7), pp.1128-1133
2018
Abstract
Anti-beta-2-glycoprotein 1 (anti-β2GP1) antibodies are associated with increased thrombotic risk in patients with autoimmune disease. There is conflicting evidence on the effects of anti-β2GP1 antibodies on platelets, with both enhanced and inhibited aggregation previously reported. However, previous studies did not include isotype antibodies to ensure the observed effects were due to anti-β2GP1 antibodies. The aims of this study were to (1) investigate the effects of anti-β2GP1 antibodies on collagen-induced platelet aggregation in parallel with negative control (buffer normal saline) and isotype control antibodies and (2) determine the lupus anticoagulant (LA) activity of anti-β2GP1 antibodies used. Three animal-derived anti-human-β2GP1 antibodies (1.25, 2.5, and 5 μg/mL) incubated with healthy platelet-rich plasma were activated by collagen (2.5 μg/mL). Each anti-β2GP1 antibody demonstrated the inhibition of aggregation compared to negative control, but not to isotype control. No anti-β2GP1 antibody demonstrated LA activity, suggesting they were probably nonpathological. This study highlights both negative and isotype control markers are important to validate the effects of anti-β2GP1 antibodies. Assays to measure anti-domain I-β2GP1 antibodies are recommended to be used in conjunction with functional measures to further investigate the effects of anti-β2GP1 antibodies.
Details
- Title
- Further investigations of the effects of anti-β2GP1 antibodies on collagen-induced platelet aggregation
- Authors/Creators
- Y.C. Ho (Author/Creator)K.D.K. Ahuja (Author/Creator)M.J. Adams (Author/Creator)
- Publication Details
- Clinical and Applied Thrombosis/Hemostasis, Vol.24(7), pp.1128-1133
- Publisher
- SAGE Publications
- Identifiers
- 991005542672707891
- Copyright
- © 2017 The Author(s)
- Murdoch Affiliation
- School of Veterinary and Life Sciences
- Language
- English
- Resource Type
- Journal article
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- Domestic collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.75 Blood Clotting
- 1.75.326 Antiphospholipid Syndrome
- Web Of Science research areas
- Hematology
- Peripheral Vascular Disease
- ESI research areas
- Clinical Medicine