Journal article
GA-20 oxidase as a candidate for the semidwarf gene sdw1/denso in barley
Functional & Integrative Genomics, Vol.9(2), pp.255-262
2009
Abstract
The barley sdw1/denso gene not only controls plant height but also yield and quality. The sdw1/denso gene was mapped to the long arm of chromosome 3H. Comparative genomic analysis revealed that the sdw1/denso gene was located in the syntenic region of the rice semidwarf gene sd1 on chromosome 1. The sd1 gene encodes a gibberellic acid (GA)-20 oxidase enzyme. The gene ortholog of rice sd1 was isolated from barley using polymerase chain reaction. The barley and rice genes showed a similar gene structure consisting of three exons and two introns. Both genes share 88.3% genomic sequence similarity and 89% amino acid sequence identity. A single nucleotide polymorphism was identified in intron 2 between barley varieties Baudin and AC Metcalfe with Baudin known to contain the denso semidwarf gene. The single nucleotide polymorphism (SNP) marker was mapped to chromosome 3H in a doubled haploid population of Baudin × AC Metcalfe with 178 DH lines. Quantitative trait locus analysis revealed that plant height cosegregated with the SNP. The sdw1/denso gene in barley is the most likely ortholog of the sd1 in rice. The result will facilitate understanding of the molecular mechanism controlling semidwarf phenotype and provide a diagnostic marker for selection of semidwarf gene in barley.
Details
- Title
- GA-20 oxidase as a candidate for the semidwarf gene sdw1/denso in barley
- Authors/Creators
- Q. Jia (Author/Creator)J. Zhang (Author/Creator)S. Westcott (Author/Creator)X-Q Zhang (Author/Creator)M. Bellgard (Author/Creator)R. Lance (Author/Creator)C. Li (Author/Creator)
- Publication Details
- Functional & Integrative Genomics, Vol.9(2), pp.255-262
- Publisher
- Springer Verlag
- Identifiers
- 991005540609707891
- Copyright
- © Springer-Verlag 2009
- Murdoch Affiliation
- Centre for Comparative Genomics; State Agricultural Biotechnology Centre
- Language
- English
- Resource Type
- Journal article
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