Gene Deletion in Barley Mediated by LTR-retrotransposon BARE

Y. Shang; F. Yang; A.H. Schulman; J. Zhu; Y. Jia; J. Wang; X-Q Zhang; Q. Jia; W. Hua; J. Yang; C. Li

doi:10.1038/srep43766

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Gene Deletion in Barley Mediated by LTR-retrotransposon BARE

Journal article

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Gene Deletion in Barley Mediated by LTR-retrotransposon BARE

Y. Shang, F. Yang, A.H. Schulman, J. Zhu, Y. Jia, J. Wang, X-Q Zhang, Q. Jia, W. Hua, J. Yang, …

Scientific Reports, Vol.7, 43766

2017

DOI: https://doi.org/10.1038/srep43766

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Abstract

A poly-row branched spike (prbs) barley mutant was obtained from soaking a two-rowed barley inflorescence in a solution of maize genomic DNA. Positional cloning and sequencing demonstrated that the prbs mutant resulted from a 28 kb deletion including the inflorescence architecture gene HvRA2. Sequence annotation revealed that the HvRA2 gene is flanked by two LTR (long terminal repeat) retrotransposons (BARE) sharing 89% sequence identity. A recombination between the integrase (IN) gene regions of the two BARE copies resulted in the formation of an intact BARE and loss of HvRA2. No maize DNA was detected in the recombination region although the flanking sequences of HvRA2 gene showed over 73% of sequence identity with repetitive sequences on 10 maize chromosomes. It is still unknown whether the interaction of retrotransposons between barley and maize has resulted in the recombination observed in the present study.

Details

Title: Gene Deletion in Barley Mediated by LTR-retrotransposon BARE
Authors/Creators: Y. Shang (Author/Creator) - ZheJiang Academy of Agricultural Sciences
F. Yang (Author/Creator) - Yangtze University
A.H. Schulman (Author/Creator) - University of Helsinki
J. Zhu (Author/Creator) - ZheJiang Academy of Agricultural Sciences
Y. Jia (Author/Creator) - Murdoch University
J. Wang (Author/Creator) - ZheJiang Academy of Agricultural Sciences
X-Q Zhang (Author/Creator) - Murdoch University
Q. Jia (Author/Creator) - Zhejiang Sci-Tech University
W. Hua (Author/Creator) - ZheJiang Academy of Agricultural Sciences
J. Yang (Author/Creator) - ZheJiang Academy of Agricultural Sciences
C. Li (Author/Creator) - Yangtze University
Publication Details: Scientific Reports, Vol.7, 43766
Publisher: Springer Nature
Identifiers: 991005545996507891
Copyright: © The Author(s) 2017
Murdoch Affiliation: School of Veterinary and Life Sciences; Western Barley Genetics Alliance
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 1 Clinical & Life Sciences; 1.54 Molecular & Cell Biology - Genetics; 1.54.1122 Transposable Elements
Web Of Science research areas: Genetics & Heredity
ESI research areas: Molecular Biology & Genetics