Genetic analysis of two common carp broodstocks by RAPD and microsatellite markers

R. Bartfai; S. Egedi; G-H Yue; B. Kovács; B. Urbányi; G. Tamás; L. Horváth; L. Orbán

doi:10.1016/S0044-8486(02)00571-9

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Genetic analysis of two common carp broodstocks by RAPD and microsatellite markers

Journal article

Peer reviewed

Genetic analysis of two common carp broodstocks by RAPD and microsatellite markers

R. Bartfai, S. Egedi, G-H Yue, B. Kovács, B. Urbányi, G. Tamás, L. Horváth and L. Orbán

Aquaculture, Vol.219(1-4), pp.157-167

2003

DOI: https://doi.org/10.1016/S0044-8486(02)00571-9

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Abstract

The whole broodstock of two Hungarian common carp farms—80 and 196 individuals—was analyzed by using random amplified polymorphic DNA (RAPD) assay and microsatellite analysis. Ten polymorphic RAPD markers and four microsatellites were selected to genotype both of the stocks. As expected, microsatellite analysis revealed more detailed information on genetic diversities than RAPD assay. Results obtained with both types of DNA markers showed lack of major differences between the genetic structure of the two stocks: heterozygosity values and allele frequencies were very similar. Dendrograms created from both sets of data did not show grouping of individuals according to stocks. Genotypes from the two stocks were also compared to those from a limited number of samples collected from other hatcheries and two rivers. Allele frequencies in the groups were similar, with the exception of wild carps. An interesting observation was that three private microsatellite alleles were found in the eight wild carp individuals, compared to the seven detected in the rest of the samples tested (372 individuals).

Details

Title: Genetic analysis of two common carp broodstocks by RAPD and microsatellite markers
Authors/Creators: R. Bartfai (Author/Creator) - Temasek Life Sciences Laboratory
S. Egedi (Author/Creator) - Agricultural Biotechnology Institute
G-H Yue (Author/Creator) - Temasek Life Sciences Laboratory
B. Kovács (Author/Creator) - Agricultural Biotechnology Institute
B. Urbányi (Author/Creator) - Magyar Agrár- és Élettudományi Egyetem
G. Tamás (Author/Creator) - Dinnyés Fish Farm, Dinnyés H-2485 Hungary
L. Horváth (Author/Creator) - Magyar Agrár- és Élettudományi Egyetem
L. Orbán (Author/Creator) - Temasek Life Sciences Laboratory
Publication Details: Aquaculture, Vol.219(1-4), pp.157-167
Publisher: Elsevier BV
Identifiers: 991005541284507891
Murdoch Affiliation: Murdoch University
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.64 Phylogenetics & Genomics; 3.64.71 Genetic Diversity
Web Of Science research areas: Fisheries; Marine & Freshwater Biology
ESI research areas: Plant & Animal Science