Abstract
Purpose
A previous study revealed that temnocephalids Diceratocephala boschmai, Craspedella pedum, and Decadidymus sp. were highly prevalent in Cheraq quadricarinatus in Melaka and Johor, Malaysia.
Methods
Herein, study was conducted to extract temnocephalids from C. quadricarinatus and identified using a molecular approach via two DNA markers: 28S ribosomal DNA and mitochondrial cytochrome c oxidase subunit I (CO1). Thereafter, the population diversity of D. boschmai in Malaysia using the CO1 DNA marker was analysed to visualize the genetic associations among populations.
Results
The analysis of 16 variable (segregating) sites in the CO1 gene alignment of sequence from D. boschmai revealed a calculated haplotype diversity of 0.1266 among populations which indicates genetic variation within the studied groups. Additionally, the determination of nucleotide diversity (II) yielded a value of 0.0035, and Tajima's D neutrality test yielded a negative value for all populations, suggesting no significant departure from neutral evolution.
Conclusion
AMOVA analysis indicated that the genetic variation or polymorphism is primarily contributed within populations (95%) compared to between populations (4%).
