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Journal article
Genetic characterization and clonal analysis of carbapenemase-producing Escherichia coli and Klebsiella pneumoniae from canine and human origins
Frontiers in veterinary science, Vol.11, 1464934
2024
PMID: 39654836
Abstract
Introduction: Carbapenem-resistant Enterobacterales (CRE), particularly carbapenemase-producing Escherichia coli and Klebsiella pneumoniae, pose a significant global health challenge due to their resistance to last-resort antibiotics. This study investigates the genetic characteristics and clonal relationships of CRE isolated from canine and human clinical samples in Bangkok to understand potential interspecies transmission.
Methods: Fifty-two CRE isolates were collected from 477 clinical samples from dogs and humans at Chulalongkorn University between 2017–2021. Bacterial species were identified using MALDI-TOF, and antimicrobial resistance was confirmed through broth microdilution testing. Genetic analyses included plasmid replicon typing, multilocus sequence typing (MLST), whole genome sequencing (WGS), and pulsed-field gel electrophoresis (PFGE) to assess resistance genes and clonal relatedness.
Results: CRE isolates from both species exhibited genetic variability with high ARG counts, particularly in human isolates. MLST identified ST410 in most E. coli isolates from both dogs and humans, and IncFIA/IncFIB plasmids were predominant among blaNDM-positive isolates. PFGE patterns and SNP analysis showed no clonal relationship between canine and human isolates, suggesting independent acquisition pathways for CRE in the two hosts.
Discussion: The study highlights the absence of direct clonal transmission between canine and human isolates but reveals overlapping sequence types and plasmid types. The findings underscore the potential for interspecies transmission under certain conditions, emphasizing the importance of a One Health approach for monitoring CRE in both human and animal populations.
Details
- Title
- Genetic characterization and clonal analysis of carbapenemase-producing Escherichia coli and Klebsiella pneumoniae from canine and human origins
- Authors/Creators
- Nwai Oo Khine - Chulalongkorn UniversityAsad Ali Shah - Chulalongkorn UniversityTanittha Chatsuwan - Chulalongkorn UniversityJitrapa Yindee - Chulalongkorn UniversityNatthapong Supimon - Chulalongkorn UniversityImporn Saenkankam - Chulalongkorn UniversityDavid John Hampson - School of Veterinary Medicine, Murdoch University, Perth, WA, AustraliaNuvee Prapasarakul - Chulalongkorn University
- Publication Details
- Frontiers in veterinary science, Vol.11, 1464934
- Publisher
- Frontiers Media S.A.
- Number of pages
- 11
- Grant note
- Chulalongkorn UniversityThailand Science Research and Innovation (TSRI), Chulalongkorn University: CU_FRB65_HEA (87)_192_31_11 National Research Council of Thailand
We are gratefully to the Pathogen Bank, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand for supporting work undertaken in this area.
- Identifiers
- 991005723863207891
- Copyright
- © 2024 Khine et al.
- Murdoch Affiliation
- School of Veterinary Medicine
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.23 Antibiotics & Antimicrobials
- 1.23.146 Antimicrobial Resistance
- Web Of Science research areas
- Veterinary Sciences
- ESI research areas
- Plant & Animal Science