Genetic characterization of cysteine-rich type-b avenin-like protein coding genes in common wheat

X.Y. Chen; X.Y. Cao; Y.J. Zhang; S. Islam; J.J. Zhang; R.C. Yang; J.J. Liu; G.Y. Li; R. Appels; G. Keeble-Gagnère; W.Q. Ji; Z.H. He; W.J. Ma

doi:10.1038/srep30692

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Genetic characterization of cysteine-rich type-b avenin-like protein coding genes in common wheat

Journal article

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Genetic characterization of cysteine-rich type-b avenin-like protein coding genes in common wheat

X.Y. Chen, X.Y. Cao, Y.J. Zhang, S. Islam, J.J. Zhang, R.C. Yang, J.J. Liu, G.Y. Li, R. Appels, G. Keeble-Gagnère, …

Scientific Reports, Vol.6, Article number: 30692

2016

DOI: https://doi.org/10.1038/srep30692

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Abstract

The wheat avenin-like proteins (ALP) are considered atypical gluten constituents and have shown positive effects on dough properties revealed using a transgenic approach. However, to date the genetic architecture of ALP genes is unclear, making it impossible to be utilized in wheat breeding. In the current study, three genes of type-b ALPs were identified and mapped to chromosomes 7AS, 4AL and 7DS. The coding gene sequence of both TaALP-7A and TaALP-7D was 855 bp long, encoding two identical homologous 284 amino acid long proteins. TaALP-4A was 858 bp long, encoding a 285 amino acid protein variant. Three alleles were identified for TaALP-7A and four for TaALP-4A. TaALP-7A alleles were of two types: type-1, which includes TaALP-7A1 andTaALP-7A2, encodes mature proteins, while type-2, represented byTaALP-7A3, contains a stop codon in the coding region and thus does not encode a mature protein. Dough quality testing of 102 wheat cultivars established a highly significant association of the type-1 TaALP-7A allele with better wheat processing quality. This allelic effects were confirmed among a range of commercial wheat cultivars. Our research makes the ALP be the first of such genetic variation source that can be readily utilized in wheat breeding.

Details

Title: Genetic characterization of cysteine-rich type-b avenin-like protein coding genes in common wheat
Authors/Creators: X.Y. Chen (Author/Creator) - Northwest A&F University
X.Y. Cao (Author/Creator)
Y.J. Zhang (Author/Creator) - Murdoch University
S. Islam (Author/Creator) - Murdoch University
J.J. Zhang (Author/Creator) - Murdoch University
R.C. Yang (Author/Creator) - Murdoch University
J.J. Liu (Author/Creator)
G.Y. Li (Author/Creator)
R. Appels (Author/Creator) - Murdoch University
G. Keeble-Gagnère (Author/Creator) - Murdoch University
W.Q. Ji (Author/Creator) - Northwest A&F University
Z.H. He (Author/Creator) - Institute of Crop Sciences
W.J. Ma (Author/Creator) - Murdoch University
Publication Details: Scientific Reports, Vol.6, Article number: 30692
Publisher: Springer Nature
Identifiers: 991005540174907891
Copyright: © 2016 Macmillan Publishers Limited
Murdoch Affiliation: Murdoch University
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.85 Food Science & Technology; 3.85.99 Cereal Starch Properties
Web Of Science research areas: Plant Sciences
ESI research areas: Agricultural Sciences