Journal article
Genetic characterization of isolates of Giardia duodenalis by enzyme electrophoresis: Implications for reproductive biology, population structure, taxonomy, and epidemiology
The Journal of Parasitology, Vol.81(3), pp.368-383
1995
Abstract
The nature and extent of genetic variation in Giardia was used to infer its mode of reproduction, population structure, taxonomy, and zoonotic potential. Ninety-seven isolates of Giardia duodenalis, from a defined area in Western Australia and throughout Australia and overseas, were obtained from humans, cats, cattle, sheep, dogs, goat, beaver, and rats. Enzyme electrophoresis revealed extensive genetic variation with 47 different zymodemes. The widespread occurrence of certain zymodemes and the similarity of relationships among isolates inferred from independent genetic markers suggests a clonal population structure for G. duodenalis, although occasional bouts of genetic exchange may occur. The 47 zymodemes clustered similarly in phenetic (UPGMA) and phylogenetic (Fitch-Margoliash) analyses. The level of genetic diversity in isolates from a defined geographical area in Western Australia was similar to the level of diversity in isolates from throughout Australia. These data suggest that clonal lineages within G. duodenalis are evolutionarily independent. Although there was a significant overall correlation between genetic distance separating zymodemes and occurrence in different host species, we found genetically identical isolates from humans and other animals and extensive genetic diversity between isolates from humans. We interpret this as evidence for zoonotic transmission of the parasite.
Details
- Title
- Genetic characterization of isolates of Giardia duodenalis by enzyme electrophoresis: Implications for reproductive biology, population structure, taxonomy, and epidemiology
- Authors/Creators
- B.P. Meloni (Author/Creator)A.J. Lymbery (Author/Creator)R.C.A. Thompson (Author/Creator)
- Publication Details
- The Journal of Parasitology, Vol.81(3), pp.368-383
- Publisher
- American Society of Parasitology
- Identifiers
- 991005542815907891
- Murdoch Affiliation
- School of Veterinary Studies
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 1 Clinical & Life Sciences
- 1.246 Diarrheal Diseases
- 1.246.985 Cryptosporidium
- Web Of Science research areas
- Parasitology
- ESI research areas
- Microbiology