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Journal article
Genetic resources and genes/QTLs for gram pod borer (Helicoverpa armigera Hübner) resistance in chickpea from the Western Himalayas
The plant genome, Vol.17(3), e20483
2024
Appears in Open Access via Read & Publish Agreements
Abstract
Helicoverpa armigera (also known as gram pod borer) is a serious threat to chickpea production in the world. A set of 173 chickpea genotypes were evaluated for H. armigera resistance, including mean larval population (MLP), percentage pod damage (PPD), and pest resistance (PR) for 2 consecutive years (year 2020 and 2021). The same core set was also genotyped with 50K Axiom CicerSNP Array. The trait data and 50,000 single nucleotide polymorphism genotypic data were used together to work out marker–trait associations (MTAs) using different genome-wide association studies models. For MLP, a total of 53 MTAs were identified, including 25 MTAs in year 2020 and 28 MTAs in year 2021. A set of three MTAs was found common in both environments. For PPD, two MTAs in year 2020 and five MTAs in year 2021 were identified. A set of two MTAs were common in both environments. Similarly, for PR, only two MTAs common in both environments were identified. Interestingly, a common MTA (Affx_123255526) on chromosome 2 (Ca2) was found to be associated with all the three component traits (MLP, PPD, and PR) of pod borer resistance in chickpea. Further, we report key genes that encode SCAMPs (that facilitates the secretion of defense-related molecules), quinone oxidoreductase (enables the production of reactive oxygen species that promotes diapause of gram pod borer), and NB-LRR proteins that have been implicated in plant defense against H. armigera. The resistant chickpea genotypes, MTAs, and key genes reported in the present study may prove useful in the future for developing pod borer–resistant chickpea varieties.
Plain Language Summary
Chickpea is one of the important crops for diet diversity in the Western Himalayas of Jammu and Kashmir. However, its production and productivity are badly challenged by the pod borer (Helicoverpa armigera). We made efforts to develop a diverse collection of chickpea and evaluated the same for pod borer resistance for 2 years. The core set of 173 chickpea genotypes was also genotyped with Axiom CicerSNP Array having ∼50,000 single nucleotide polymorphisms. The analysis of trait data and genotypic data together led to the identification of genes/quantitative trait loci (QTLs) for pod borer resistance. A total of 53 significant marker trait associations/genes/QTLs conferring resistance to H. armigera (mean larval population) were identified. Candidate gene analysis identified key genes responsible for plant defense against H. armigera in Chickpea. The study will prove useful for chickpea breeding programs in the world. The genes identified will prove useful in developing pod borer–resistant chickpea varieties.
Details
- Title
- Genetic resources and genes/QTLs for gram pod borer (Helicoverpa armigera Hübner) resistance in chickpea from the Western Himalayas
- Authors/Creators
- Sheikh Aafreen Rehman - Sher-e-Kashmir University of Agricultural Sciences and Technology of KashmirShaheen Gul - Sher-e-Kashmir University of Agricultural Sciences and Technology of KashmirM Parthiban - Sher-e-Kashmir University of Agricultural Sciences and Technology of KashmirIshita Isha - Central Agricultural UniversityM S Sai Reddy - Central Agricultural UniversityAnnapurna Chitikineni - Murdoch UniversityMahendar Thudi - Central Agricultural UniversityR Varma Penmetsa - University of California, DavisRajeev Kumar Varshney - Murdoch UniversityReyazul Rouf Mir - Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir
- Publication Details
- The plant genome, Vol.17(3), e20483
- Identifiers
- 991005684770007891
- Copyright
- © 2024 The Author(s)
- Murdoch Affiliation
- Food Futures Institute
- Language
- English
- Resource Type
- Journal article
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