Genetic variability of Trypanosoma evansi isolates detected by inter-simple sequence repeat anchored-PCR and microsatellite

Z. Njiru; C.C. Constantine; P. Gitonga; R.C.A. Thompson; S.A. Reid

doi:10.1016/j.vetpar.2007.03.010

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Genetic variability of Trypanosoma evansi isolates detected by inter-simple sequence repeat anchored-PCR and microsatellite

Journal article

Peer reviewed

Genetic variability of Trypanosoma evansi isolates detected by inter-simple sequence repeat anchored-PCR and microsatellite

Z. Njiru, C.C. Constantine, P. Gitonga, R.C.A. Thompson and S.A. Reid

Veterinary Parasitology, Vol.147(1-2), pp.51-60

2007

DOI: https://doi.org/10.1016/j.vetpar.2007.03.010

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Abstract

Studies on genetic variability in Trypanosoma evansi have been limited by a lack of high-resolution techniques. In this study, we have investigated the use of inter-simple sequence repeats (ISSR) and microsatellites in revealing polymorphism among T. evansi isolates. Twelve ISSR primers and five microsatellite loci were used to generate polymorphic bands and alleles, respectively, to investigate the genetic variability among T. evansi isolates from Africa and Asia. Seven of the twelve ISSR primers showed variability between isolates with a total of 71 fragments of which 49(69%) were polymorphic. Microsatellite analysis revealed a total of 60 alleles. On average the ISSR markers revealed a higher genetic diversity (23%) than microsatellites (21.1%). The two techniques showed a strong agreement of r = 0.95 for Dice and r = 0.91 for Jaccard indices in estimating the genetic distances between isolates. The distance UPGMA tree revealed two major clusters of T. evansi which correlate with the minicircle classification of subtype A and B. The cophenetic correlation coefficient between Dice and Jaccard based matrices were r = 0.79 for microsatellites and r = 0.73 for ISSR indicating a strong agreement between dendrograms. The results suggest that both ISSR and microsatellites markers are useful in detecting genetic variability within T. evansi.

Details

Title: Genetic variability of Trypanosoma evansi isolates detected by inter-simple sequence repeat anchored-PCR and microsatellite
Authors/Creators: Z. Njiru (Author/Creator)
C.C. Constantine (Author/Creator)
P. Gitonga (Author/Creator)
R.C.A. Thompson (Author/Creator)
S.A. Reid (Author/Creator)
Publication Details: Veterinary Parasitology, Vol.147(1-2), pp.51-60
Publisher: Elsevier BV
Identifiers: 991005544685407891
Copyright: © 2007 Elsevier B.V.
Murdoch Affiliation: School of Veterinary and Biomedical Sciences; School of Nursing and Midwifery
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 1 Clinical & Life Sciences; 1.261 Parasitology - Trypanosoma & Leishmania; 1.261.596 Trypanosoma Biology
Web Of Science research areas: Parasitology; Veterinary Sciences
ESI research areas: Plant & Animal Science