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Genome-Wide discovery and deployment of insertions and deletions markers provided greater insights on species, genomes, and sections relationships in the Genus Arachis
Journal article   Open access   Peer reviewed

Genome-Wide discovery and deployment of insertions and deletions markers provided greater insights on species, genomes, and sections relationships in the Genus Arachis

M.K. Vishwakarma, S.M. Kale, M. Sriswathi, T. Naresh, Y. Shasidhar, V. Garg, M.K. Pandey and R.K. Varshney
Frontiers in Plant Science, Vol.8, Art. 02064
2017
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Abstract

Small insertions and deletions (InDels) are the second most prevalent and the most abundant structural variations in plant genomes. In order to deploy these genetic variations for genetic analysis in genus Arachis, we conducted comparative analysis of the draft genome assemblies of both the diploid progenitor species of cultivated tetraploid groundnut (Arachis hypogaea L.) i.e., Arachis duranensis (A subgenome) and Arachis ipaënsis (B subgenome) and identified 515,223 InDels. These InDels include 269,973 insertions identified in A. ipaënsis against A. duranensis while 245,250 deletions in A. duranensis against A. ipaënsis. The majority of the InDels were of single bp (43.7%) and 2–10 bp (39.9%) while the remaining were >10 bp (16.4%). Phylogenetic analysis using genotyping data for 86 (40.19%) polymorphic markers grouped 96 diverse Arachis accessions into eight clusters mostly by the affinity of their genome. This study also provided evidence for the existence of “K” genome, although distinct from both the “A” and “B” genomes, but more similar to “B” genome. The complete homology between A. monticola and A. hypogaea tetraploid taxa showed a very similar genome composition. The above analysis has provided greater insights into the phylogenetic relationship among accessions, genomes, sub species and sections. These InDel markers are very useful resource for groundnut research community for genetic analysis and breeding applications.

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Collaboration types
Domestic collaboration
International collaboration
Citation topics
3 Agriculture, Environment & Ecology
3.4 Crop Science
3.4.96 QTL
Web Of Science research areas
Plant Sciences
ESI research areas
Plant & Animal Science
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