Genome-Wide identification and analysis of the Hsp40/J-Protein family reveals its role in Soybean (Glycine max) growth and development

Muhammad Khuram Razzaq; Reena Rani; Guangnan Xing; Yufei Xu; Ghulam Raza; Muqadas Aleem; Shahid Iqbal; Muhammad Arif; Zahid Mukhtar; Henry T. Nguyen; Rajeev K. Varshney; Kadambot H. M. Siddique; Junyi Gai

doi:10.3390/genes14061254

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Genome-Wide identification and analysis of the Hsp40/J-Protein family reveals its role in Soybean (Glycine max) growth and development

Journal article

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Genome-Wide identification and analysis of the Hsp40/J-Protein family reveals its role in Soybean (Glycine max) growth and development

Muhammad Khuram Razzaq, Reena Rani, Guangnan Xing, Yufei Xu, Ghulam Raza, Muqadas Aleem, Shahid Iqbal, Muhammad Arif, Zahid Mukhtar, Henry T. Nguyen, …

Genes, Vol.14(6), 1254

2023

DOI: https://doi.org/10.3390/genes14061254

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Abstract

Glycine max (L.) Merr

growth period

Hsp40

J-protein characterization

J-protein family

seed development

The J-protein family comprises molecular chaperones involved in plant growth, development, and stress responses. Little is known about this gene family in soybean. Hence, we characterized J-protein genes in soybean, with the most highly expressed and responsive during flower and seed development. We also revealed their phylogeny, structure, motif analysis, chromosome location, and expression. Based on their evolutionary links, we divided the 111 potential soybean J-proteins into 12 main clades (I–XII). Gene-structure estimation revealed that each clade had an exon-intron structure resembling or comparable to others. Most soybean J-protein genes lacked introns in Clades I, III, and XII. Moreover, transcriptome data obtained from a publicly accessible soybean database and RT-qPCR were used to examine the differential expression of DnaJ genes in various soybean tissues and organs. The expression level of DnaJ genes indicated that, among 14 tissues, at least one tissue expressed the 91 soybean genes. The findings suggest that J-protein genes could be involved in the soybean growth period and offer a baseline for further functional research into J-proteins' role in soybean. One important application is the identification of J-proteins that are highly expressed and responsive during flower and seed development in soybean. These genes likely play crucial roles in these processes, and their identification can contribute to breeding programs to improve soybean yield and quality.

Details

Title: Genome-Wide identification and analysis of the Hsp40/J-Protein family reveals its role in Soybean (Glycine max) growth and development
Authors/Creators: Muhammad Khuram Razzaq - Nanjing Agricultural University
Reena Rani - National Institute for Biotechnology and Genetic Engineering
Guangnan Xing - Nanjing Agricultural University
Yufei Xu - Nanjing Agricultural University
Ghulam Raza - National Institute for Biotechnology and Genetic Engineering
Muqadas Aleem - University of Agriculture Faisalabad
Shahid Iqbal - University of Florida
Muhammad Arif - National Institute for Biotechnology and Genetic Engineering
Zahid Mukhtar - National Institute for Biotechnology and Genetic Engineering
Henry T. Nguyen - University of Missouri
Rajeev K. Varshney - Murdoch University, Food Futures Institute
Kadambot H. M. Siddique - The University of Western Australia
Junyi Gai - Nanjing Agricultural University
Publication Details: Genes, Vol.14(6), 1254
Publisher: MDPI
Grant note: University of Western Australia
Identifiers: 991005589869007891
Murdoch Affiliation: Centre for Crop and Food Innovation; Food Futures Institute; State Agricultural Biotechnology Centre
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 1 Clinical & Life Sciences; 1.25 Molecular & Cell Biology - Cancer, Autophagy & Apoptosis; 1.25.512 Heat Shock Proteins
Web Of Science research areas: Genetics & Heredity
ESI research areas: Molecular Biology & Genetics