Genome-Wide investigation of Apyrase (APY) genes in peanut (Arachis hypogaea L.) and functional characterization of a Pod-Abundant expression promoter AhAPY2-1p

Yasir Sharif; Gandeka Mamadou; Qiang Yang; Tiecheng Cai; Yuhui Zhuang; Kun Chen; Ye Deng; Shahid Ali Khan; Niaz Ali; Chong Zhang; Ali Raza; Hua Chen; Rajeev K. Varshney; Weijian Zhuang

doi:10.3390/ijms24054622

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Genome-Wide investigation of Apyrase (APY) genes in peanut (Arachis hypogaea L.) and functional characterization of a Pod-Abundant expression promoter AhAPY2-1p

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Genome-Wide investigation of Apyrase (APY) genes in peanut (Arachis hypogaea L.) and functional characterization of a Pod-Abundant expression promoter AhAPY2-1p

Yasir Sharif, Gandeka Mamadou, Qiang Yang, Tiecheng Cai, Yuhui Zhuang, Kun Chen, Ye Deng, Shahid Ali Khan, Niaz Ali, Chong Zhang, …

International journal of molecular sciences, Vol.24(5), Art. 4622

2023

DOI: https://doi.org/10.3390/ijms24054622

PMID: 36902052

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Abstract

Biochemistry & Molecular Biology

Chemistry

Chemistry, Multidisciplinary

Life Sciences & Biomedicine

Physical Sciences

Science & Technology

Peanut (Arachis hypogaea L.) is an important food and feed crop worldwide and is affected by various biotic and abiotic stresses. The cellular ATP levels decrease significantly during stress as ATP molecules move to extracellular spaces, resulting in increased ROS production and cell apoptosis. Apyrases (APYs) are the nucleoside phosphatase (NPTs) superfamily members and play an important role in regulating cellular ATP levels under stress. We identified 17 APY homologs in A. hypogaea (AhAPYs), and their phylogenetic relationships, conserved motifs, putative miRNAs targeting different AhAPYs, cis-regulatory elements, etc., were studied in detail. The transcriptome expression data were used to observe the expression patterns in different tissues and under stress conditions. We found that the AhAPY2-1 gene showed abundant expression in the pericarp. As the pericarp is a key defense organ against environmental stress and promoters are the key elements regulating gene expression, we functionally characterized the AhAPY2-1 promoter for its possible use in future breeding programs. The functional characterization of AhAPY2-1P in transgenic Arabidopsis plants showed that it effectively regulated GUS gene expression in the pericarp. GUS expression was also detected in flowers of transgenic Arabidopsis plants. Overall, these results strongly suggest that APYs are an important future research subject for peanut and other crops, and AhPAY2-1P can be used to drive the resistance-related genes in a pericarp-specific manner to enhance the defensive abilities of the pericarp.

Details

Title: Genome-Wide investigation of Apyrase (APY) genes in peanut (Arachis hypogaea L.) and functional characterization of a Pod-Abundant expression promoter AhAPY2-1p
Authors/Creators: Yasir Sharif - Fujian Agr & Forestry Univ FAFU, Inst Oil Crops, Ctr Legume Plant Genet & Syst Biol, Coll Agr, Fuzhou 350002, Peoples R China
Gandeka Mamadou - Fujian Agriculture and Forestry University
Qiang Yang - Fujian Agriculture and Forestry University
Tiecheng Cai - Fujian Agriculture and Forestry University
Yuhui Zhuang - Fujian Agr & Forestry Univ, Coll Life Sci, Fuzhou 350002, Peoples R China
Kun Chen - Fujian Agriculture and Forestry University
Ye Deng - Fujian Agriculture and Forestry University
Shahid Ali Khan - Plant (United States)
Niaz Ali - Fujian Agriculture and Forestry University
Chong Zhang - Fujian Agriculture and Forestry University
Ali Raza - Fujian Agr & Forestry Univ FAFU, Inst Oil Crops, Ctr Legume Plant Genet & Syst Biol, Coll Agr, Fuzhou 350002, Peoples R China
Hua Chen - Fujian Agriculture and Forestry University
Rajeev K. Varshney - Murdoch University, Food Futures Institute
Weijian Zhuang - Fujian Agriculture and Forestry University
Publication Details: International journal of molecular sciences, Vol.24(5), Art. 4622
Publisher: MDPI
Number of pages: 25
Grant note: 2021N5007; 2017N0006 / Science and Technology Foundation of Fujian Province of China KFb22010XA; KFb22011XA / Special Fund for Scientific and Technological Innovation of Fujian Agriculture and Forestry University U1705233; 32272155; 32072103; 31701463; 31601337 / National Natural Science Foundation (NSFC) of China; National Natural Science Foundation of China (NSFC)
Identifiers: 991005566470007891
Murdoch Affiliation: Centre for Crop and Food Innovation; State Agricultural Biotechnology Centre
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 1 Clinical & Life Sciences; 1.159 Membrane Channels & Receptors; 1.159.576 Adenosine Signaling
Web Of Science research areas: Biochemistry & Molecular Biology; Chemistry, Multidisciplinary
ESI research areas: Chemistry