Journal article
Genome-based analysis of the transcriptome from mature chickpea root nodules
Frontiers in Plant Science, Vol.5, Art. 00325
2014
Abstract
Symbiotic nitrogen fixation (SNF) in root nodules of grain legumes such as chickpea is a highly complex process that drastically affects the gene expression patterns of both the prokaryotic as well as eukaryotic interacting cells. A successfully established symbiotic relationship requires mutual signaling mechanisms and a continuous adaptation of the metabolism of the involved cells to varying environmental conditions. Although some of these processes are well understood today many of the molecular mechanisms underlying SNF, especially in chickpea, remain unclear. Here, we reannotated our previously published transcriptome data generated by deepSuperSAGE (Serial Analysis of Gene Expression) to the recently published draft genome of chickpea to assess the root- and nodule-specific transcriptomes of the eukaryotic host cells. The identified gene expression patterns comprise up to 71 significantly differentially expressed genes and the expression of twenty of these was validated by quantitative real-time PCR with the tissues from five independent biological replicates. Many of the differentially expressed transcripts were found to encode proteins implicated in sugar metabolism, antioxidant defense as well as biotic and abiotic stress responses of the host cells, and some of them were already known to contribute to SNF in other legumes. The differentially expressed genes identified in this study represent candidates that can be used for further characterization of the complex molecular mechanisms underlying SNF in chickpea.
Details
- Title
- Genome-based analysis of the transcriptome from mature chickpea root nodules
- Authors/Creators
- F. Afonso-Grunz (Author/Creator) - Goethe University FrankfurtC. Molina (Author/Creator) - Christian-Albrechts-Universität zu KielK. Hoffmeier (Author/Creator) - GenXPro (Germany)L. Rycak (Author/Creator) - GenXPro (Germany)H. Kudapa (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsR.K. Varshney (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsJ-J Drevon (Author/Creator) - Institut National de la Recherche AgronomiqueP. Winter (Author/Creator) - GenXPro (Germany)G. Kahl (Author/Creator) - Goethe University Frankfurt
- Publication Details
- Frontiers in Plant Science, Vol.5, Art. 00325
- Publisher
- Frontiers
- Identifiers
- 991005542788407891
- Copyright
- © 2021 The Authors.
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.97 Plant Pathology
- 3.97.892 Rhizobium-Legume Symbiosis
- Web Of Science research areas
- Plant Sciences
- ESI research areas
- Plant & Animal Science