Genome sequencing and analysis of the paclitaxel-producing endophytic fungus Penicillium aurantiogriseum NRRL 62431

Y. Yang; H. Zhao; R.A. Barrero; B. Zhang; G. Sun; I.W. Wilson; F. Xie; K.D. Walker; J.W. Parks; R. Bruce; G. Guo; L. Chen; Y. Zhang; X. Huang; Qi Tang; H. Liu; M.I. Bellgard; D. Qiu; J. Lai; A. Hoffman

doi:10.1186/1471-2164-15-69

Back

Genome sequencing and analysis of the paclitaxel-producing endophytic fungus Penicillium aurantiogriseum NRRL 62431

Journal article

Open access

Peer reviewed

Genome sequencing and analysis of the paclitaxel-producing endophytic fungus Penicillium aurantiogriseum NRRL 62431

Y. Yang, H. Zhao, R.A. Barrero, B. Zhang, G. Sun, I.W. Wilson, F. Xie, K.D. Walker, J.W. Parks, R. Bruce, …

BMC Genomics, Vol.15(1)

2014

DOI: https://doi.org/10.1186/1471-2164-15-69

Files and links (2)

pdf

genome_sequencing.pdfDownload View

Published (Version of Record) Open Access

url

Free to Read *No subscription requiredView

Abstract

Background Paclitaxel (Taxol™) is an important anticancer drug with a unique mode of action. The biosynthesis of paclitaxel had been considered restricted to the Taxus species until it was discovered in Taxomyces andreanae, an endophytic fungus of T. brevifolia. Subsequently, paclitaxel was found in hazel (Corylus avellana L.) and in several other endophytic fungi. The distribution of paclitaxel in plants and endophytic fungi and the reported sequence homology of key genes in paclitaxel biosynthesis between plant and fungi species raises the question about whether the origin of this pathway in these two physically associated groups could have been facilitated by horizontal gene transfer. Results The ability of the endophytic fungus of hazel Penicillium aurantiogriseum NRRL 62431 to independently synthesize paclitaxel was established by liquid chromatography-mass spectrometry and proton nuclear magnetic resonance. The genome of Penicillium aurantiogriseum NRRL 62431 was sequenced and gene candidates that may be involved in paclitaxel biosynthesis were identified by comparison with the 13 known paclitaxel biosynthetic genes in Taxus. We found that paclitaxel biosynthetic gene candidates in P. aurantiogriseum NRRL 62431 have evolved independently and that horizontal gene transfer between this endophytic fungus and its plant host is unlikely. Conclusions Our findings shed new light on how paclitaxel-producing endophytic fungi synthesize paclitaxel, and will facilitate metabolic engineering for the industrial production of paclitaxel from fungi.

Details

Title: Genome sequencing and analysis of the paclitaxel-producing endophytic fungus Penicillium aurantiogriseum NRRL 62431
Authors/Creators: Y. Yang (Author/Creator) - Research Institute of Forestry
H. Zhao (Author/Creator) - China Agricultural University
R.A. Barrero (Author/Creator) - Murdoch University
B. Zhang (Author/Creator) - East Carolina University
G. Sun (Author/Creator) - Kunming Institute of Botany
I.W. Wilson (Author/Creator) - Plant Industry
F. Xie (Author/Creator) - East Carolina University
K.D. Walker (Author/Creator) - Michigan State University
J.W. Parks (Author/Creator) - University of Portland
R. Bruce (Author/Creator) - University of Portland
G. Guo (Author/Creator) - BGI (United States)
L. Chen (Author/Creator) - BGI (United States)
Y. Zhang (Author/Creator) - China Agricultural University
X. Huang (Author/Creator) - Chinese Academy of Inspection and Quarantine
Qi Tang (Author/Creator) - Hunan Agricultural University
H. Liu (Author/Creator) - Research Institute of Forestry
M.I. Bellgard (Author/Creator) - Murdoch University
D. Qiu (Author/Creator) - Research Institute of Forestry
J. Lai (Author/Creator) - China Agricultural University
A. Hoffman (Author/Creator) - University of Portland
Publication Details: BMC Genomics, Vol.15(1)
Publisher: BioMed Central
Identifiers: 991005541529507891
Copyright: © 2014 Yang et al.
Murdoch Affiliation: Centre for Comparative Genomics
Language: English
Resource Type: Journal article

UN Sustainable Development Goals (SDGs)

This output has contributed to the advancement of the following goals:

Source: InCites

Metrics

184 File views/ downloads

77 Record Views

114 Times Cited - Web of Science

InCites Highlights

These are selected metrics from InCites Benchmarking & Analytics tool, related to this output

Collaboration types: Domestic collaboration; International collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.198 Mycotoxins; 3.198.926 Endophytic Fungi
Web Of Science research areas: Biotechnology & Applied Microbiology; Genetics & Heredity
ESI research areas: Molecular Biology & Genetics