Journal article
Genome-wide association study and identification of candidate genes for nitrogen use efficiency in barley (Hordeum vulgare L.)
Frontiers in Plant Science, Vol.11, Article 571912
2020
Abstract
Nitrogen (N) fertilizer is largely responsible for barley grain yield potential and quality, yet excessive application leads to environmental pollution and high production costs. Therefore, efficient use of N is fundamental for sustainable agriculture. In the present study, we investigated the performance of 282 barley accessions through hydroponic screening using optimal and low NH4NO3 treatments. Low-N treatment led to an average shoot dry weight reduction of 50%, but there were significant genotypic differences among the accessions. Approximately 20% of the genotypes showed high (>75%) relative shoot dry weight under low-N treatment and were classified as low-N tolerant, whereas 20% were low-N sensitive (≤55%). Low-N tolerant accessions exhibited well-developed root systems with an average increase of 60% in relative root dry weight to facilitate more N absorption. A genome-wide association study (GWAS) identified 66 significant marker trait associations (MTAs) conferring high nitrogen use efficiency, four of which were stable across experiments. These four MTAs were located on chromosomes 1H(1), 3H(1), and 7H(2) and were associated with relative shoot length, relative shoot and root dry weight. Genes corresponding to the significant MTAs were retrieved as candidate genes, including members of the asparagine synthetase gene family, several transcription factor families, protein kinases, and nitrate transporters. Most importantly, the high-affinity nitrate transporter 2.7 (HvNRT2.7) was identified as a promising candidate on 7H for root and shoot dry weight. The identified candidate genes provide new insights into our understanding of the molecular mechanisms driving nitrogen use efficiency in barley and represent potential targets for genetic improvement.
Details
- Title
- Genome-wide association study and identification of candidate genes for nitrogen use efficiency in barley (Hordeum vulgare L.)
- Authors/Creators
- S.D. Karunarathne (Author/Creator) - Murdoch UniversityY. Han (Author/Creator) - Murdoch UniversityX-Q Zhang (Author/Creator) - Murdoch UniversityG. Zhou (Author/Creator) - Government of Western AustraliaC.B. Hill (Author/Creator) - Murdoch UniversityK. Chen (Author/Creator) - Curtin UniversityT. Angessa (Author/Creator) - Murdoch UniversityC. Li (Author/Creator) - Government of Western Australia
- Publication Details
- Frontiers in Plant Science, Vol.11, Article 571912
- Publisher
- Frontiers Media
- Identifiers
- 991005542950007891
- Copyright
- © 2020 Karunarathne, Han, Zhang, Zhou, Hill, Chen, Angessa and Li
- Murdoch Affiliation
- College of Science, Health, Engineering and Education; State Agricultural Biotechnology Centre; Western Barley Genetics Alliance
- Language
- English
- Resource Type
- Journal article
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Source: InCites
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- Collaboration types
- Domestic collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.4 Crop Science
- 3.4.1637 Plant Nitrogen Metabolism
- Web Of Science research areas
- Plant Sciences
- ESI research areas
- Plant & Animal Science