Genome-wide identification and analysis of non-specific Lipid Transfer Proteins in hexaploid wheat

Allan Kouidri; Ryan Whitford; Radoslaw Suchecki; Elena Kalashyan; Ute Baumann

doi:10.1038/s41598-018-35375-7

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Genome-wide identification and analysis of non-specific Lipid Transfer Proteins in hexaploid wheat

Journal article

Open access

Peer reviewed

Genome-wide identification and analysis of non-specific Lipid Transfer Proteins in hexaploid wheat

Allan Kouidri, Ryan Whitford, Radoslaw Suchecki, Elena Kalashyan and Ute Baumann

Scientific reports, Vol.8(1), 17087

2018

DOI: https://doi.org/10.1038/s41598-018-35375-7

PMID: 30459322

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Abstract

Carrier Proteins - analysis

Carrier Proteins - metabolism

Gene Expression Regulation, Plant

Genome, Plant

Phylogeny

Plant Proteins - analysis

Plant Proteins - metabolism

Triticum - genetics

Triticum - growth & development

Triticum - metabolism

Non-specific Lipid Transfer Proteins (nsLTPs) are involved in numerous biological processes. To date, only a fraction of wheat (Triticum aestivum L.) nsLTPs (TaLTPs) have been identified, and even fewer have been functionally analysed. In this study, the identification, classification, phylogenetic reconstruction, chromosome distribution, functional annotation and expression profiles of TaLTPs were analysed. 461 putative TaLTPs were identified from the wheat genome and classified into five types (1, 2, C, D and G). Phylogenetic analysis of the TaLTPs along with nsLTPs from Arabidopsis thaliana and rice, showed that all five types were shared across species, however, some type 2 TaLTPs formed wheat-specific clades. Gene duplication analysis indicated that tandem duplications contributed to the expansion of this gene family in wheat. Analysis of RNA sequencing data showed that TaLTPs were expressed in most tissues and stages of wheat development. Further, we refined the expression profile of anther-enriched expressed genes, and identified potential cis-elements regulating their expression specificity. This analysis provides a valuable resource towards elucidating the function of TaLTP family members during wheat development, aids our understanding of the evolution and expansion of the TaLTP gene family and, additionally, provides new information for developing wheat male-sterile lines with application to hybrid breeding.

Details

Title: Genome-wide identification and analysis of non-specific Lipid Transfer Proteins in hexaploid wheat
Authors/Creators: Allan Kouidri - The University of Adelaide
Ryan Whitford - The University of Adelaide
Radoslaw Suchecki - Commonwealth Scientific and Industrial Research Organization, Agriculture and Food, Waite Campus, Urrbrae, South Australia, 5064, Australia
Elena Kalashyan - The University of Adelaide
Ute Baumann - The University of Adelaide
Publication Details: Scientific reports, Vol.8(1), 17087
Publisher: Springer Nature
Number of pages: 12
Identifiers: 991005779528907891
Murdoch Affiliation: Centre for Crop and Food Innovation
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.4 Crop Science; 3.4.96 QTL
Web Of Science research areas: Plant Sciences
ESI research areas: Plant & Animal Science