Genome-wide survey and expression analysis of F-box genes in chickpea

Shefali Gupta; Vanika Garg; Chandra Kant; Sabhyata Bhatia

doi:10.1186/s12864-015-1293-y

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Genome-wide survey and expression analysis of F-box genes in chickpea

Journal article

Open access

Peer reviewed

Genome-wide survey and expression analysis of F-box genes in chickpea

Shefali Gupta, Vanika Garg, Chandra Kant and Sabhyata Bhatia

BMC genomics, Vol.16(1), 67

2015

DOI: https://doi.org/10.1186/s12864-015-1293-y

PMCID: PMC4340835

PMID: 25759239

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Abstract

Biotechnology & Applied Microbiology

Genetics & Heredity

Life Sciences & Biomedicine

Science & Technology

Background: The F-box genes constitute one of the largest gene families in plants involved in degradation of cellular proteins. F-box proteins can recognize a wide array of substrates and regulate many important biological processes such as embryogenesis, floral development, plant growth and development, biotic and abiotic stress, hormonal responses and senescence, among others. However, little is known about the F-box genes in the important legume crop, chickpea. The available draft genome sequence of chickpea allowed us to conduct a genome-wide survey of the F-box gene family in chickpea. Results: A total of 285 F-box genes were identified in chickpea which were classified based on their C-terminal domain structures into 10 subfamilies. Thirteen putative novel motifs were also identified in F-box proteins with no known functional domain at their C-termini. The F-box genes were physically mapped on the 8 chickpea chromosomes and duplication events were investigated which revealed that the F-box gene family expanded largely due to tandem duplications. Phylogenetic analysis classified the chickpea F-box genes into 9 clusters. Also, maximum syntenic relationship was observed with soybean followed by Medicago truncatula, Lotus japonicus and Arabidopsis. Digital expression analysis of F-box genes in various chickpea tissues as well as under abiotic stress conditions utilizing the available chickpea transcriptome data revealed differential expression patterns with several F-box genes specifically expressing in each tissue, few of which were validated by using quantitative real-time PCR. Conclusions: The genome-wide analysis of chickpea F-box genes provides new opportunities for characterization of candidate F-box genes and elucidation of their function in growth, development and stress responses for utilization in chickpea improvement.

Details

Title: Genome-wide survey and expression analysis of F-box genes in chickpea
Authors/Creators: Shefali Gupta - Natl Inst Plant Genome Res, New Delhi 110067, India
Vanika Garg
Chandra Kant - Natl Inst Plant Genome Res, New Delhi 110067, India
Sabhyata Bhatia - Natl Inst Plant Genome Res, New Delhi 110067, India
Publication Details: BMC genomics, Vol.16(1), 67
Publisher: Springer Nature
Identifiers: 991005579266807891
Murdoch Affiliation: Murdoch University
Language: English
Resource Type: Journal article

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Citation topics: 3 Agriculture, Environment & Ecology; 3.4 Crop Science; 3.4.159 Arabidopsis
Web Of Science research areas: Biotechnology & Applied Microbiology; Genetics & Heredity
ESI research areas: Molecular Biology & Genetics