Genomic analysis of phylogenetic group B2 extraintestinal pathogenic E. coli causing infections in dogs in Australia

A.K. Kidsley; M. O’Dea; S. Saputra; D. Jordan; J.R. Johnson; D.M. Gordon; C. Turni; S.P. Djordjevic; S. Abraham; D.J. Trott

doi:10.1016/j.vetmic.2020.108783

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Genomic analysis of phylogenetic group B2 extraintestinal pathogenic E. coli causing infections in dogs in Australia

Journal article

Peer reviewed

Genomic analysis of phylogenetic group B2 extraintestinal pathogenic E. coli causing infections in dogs in Australia

A.K. Kidsley, M. O’Dea, S. Saputra, D. Jordan, J.R. Johnson, D.M. Gordon, C. Turni, S.P. Djordjevic, S. Abraham and D.J. Trott

Veterinary Microbiology, Vol.248, Art.108783

2020

DOI: https://doi.org/10.1016/j.vetmic.2020.108783

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Abstract

This study investigated the prevalence of extraintestinal pathogenic E. coli (ExPEC)-associated sequence types (STs) from phylogenetic group B2 among 449 fluoroquinolone-susceptible dog clinical isolates from Australia. Isolates underwent PCR-based phylotyping and random amplified polymorphic DNA analysis to determine clonal relatedness. Of the 317 so-identified group B2 isolates, 77 underwent whole genome sequencing (WGS), whereas the remainder underwent PCR-based screening for ST complexes (STc) STc12, STc73, STc372, and ST131. The predominant ST was ST372 according to both WGS (31 % of 77) and ST-specific PCR (22 % of 240), followed by (per WGS) ST73 (17 %), ST12 (7 %), and ST80 (7 %). A WGS-based phylogenetic comparison of ST73 isolates from dogs, cats, and humans showed considerable overall phylogenetic diversity. Although most clusters were species-specific, some contained closely related human and animal (dog > cat) isolates. For dogs in Australia these findings both confirm ST372 as the predominant E. coli clonal lineage causing extraintestinal infections and clarify the importance of human-associated group B2 lineage ST73 as a cause of UTI, with some strains possibly being capable of bi-directional (i.e., dog-human and human-dog) transmission.

Details

Title: Genomic analysis of phylogenetic group B2 extraintestinal pathogenic E. coli causing infections in dogs in Australia
Authors/Creators: A.K. Kidsley (Author/Creator) - The University of Adelaide
M. O’Dea (Author/Creator) - Murdoch University
S. Saputra (Author/Creator) - The University of Adelaide
D. Jordan (Author/Creator) - New South Wales Department of Primary Industries
J.R. Johnson (Author/Creator) - Minneapolis VA Medical Center
D.M. Gordon (Author/Creator) - Australian National University
C. Turni (Author/Creator)
S.P. Djordjevic (Author/Creator) - University of Technology Sydney
S. Abraham (Author/Creator) - Murdoch University
D.J. Trott (Author/Creator) - The University of Adelaide
Publication Details: Veterinary Microbiology, Vol.248, Art.108783
Publisher: Elsevier BV
Identifiers: 991005544928307891
Copyright: © 2020 Elsevier B.V.
Murdoch Affiliation: College of Science, Health, Engineering and Education; Antimicrobial Resistance and Infectious Disease Laboratory
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 1 Clinical & Life Sciences; 1.23 Antibiotics & Antimicrobials; 1.23.146 Antimicrobial Resistance
Web Of Science research areas: Microbiology; Veterinary Sciences
ESI research areas: Plant & Animal Science