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Journal article
Genomic characterization of a unique Panton-Valentine leucocidin-positive community-associated methicillin-resistant Staphylococcus aureus lineage increasingly impacting on Australian indigenous communities
Microbial genomics, Vol.9(12), 001172
2023
PMCID: PMC10763498
PMID: 38117559
Abstract
In 2010 a single isolate of a trimethoprim-resistant multilocus sequence type 5, Panton-Valentine leucocidin-positive, community-associated methicillin-resistant
(PVL-positive ST5 CA-MRSA), colloquially named WA121, was identified in northern Western Australia (WA). WA121 now accounts for ~14 % of all WA MRSA infections. To gain an understanding of the genetic composition and phylogenomic structure of WA121 isolates we sequenced the genomes of 155 WA121 isolates collected 2010-2021 and present a detailed genomic description. WA121 was revealed to be a single clonally expanding lineage clearly distinct from sequenced ST5 strains reported outside Australia. WA121 strains were typified by the presence of the distinct PVL phage φSa2wa-st5, the recently described methicillin resistance element SCC
IVo carrying the trimethoprim resistance (
) transposon Tn
, the novel β-lactamase transposon Tn
and the epidermal cell differentiation inhibitor (EDIN-A) plasmid p2010-15611-2. We present evidence that SCC
IVo together with Tn
has horizontally transferred to
and evidence of intragenomic movement of both Tn
and Tn
. We experimentally demonstrate that p2010-15611-2 is capable of horizontal transfer by conjugative mobilization from one of several WA121 isolates also harbouring a pWBG749-like conjugative plasmid. In summary, WA121 is a distinct and clonally expanding Australian PVL-positive CA-MRSA lineage that is increasingly responsible for infections in indigenous communities in northern and western Australia. WA121 harbours a unique complement of mobile genetic elements and is capable of transferring antimicrobial resistance and virulence determinants to other staphylococci.
Details
- Title
- Genomic characterization of a unique Panton-Valentine leucocidin-positive community-associated methicillin-resistant Staphylococcus aureus lineage increasingly impacting on Australian indigenous communities
- Authors/Creators
- Joshua P Ramsay - Curtin UniversityNipuna Parahitiyawa - Curtin UniversityShakeel Mowlaboccus - Murdoch UniversityChristopher A Mullally - Murdoch UniversityNicholas W T Yee - Antimicrobial Resistance and Infectious Disease (AMRID) Research Laboratory, College of Science, Health, Engineering and Education, Murdoch University, Perth, WA, AustraliaPrincy Shoby - Murdoch UniversityElena Colombi - Curtin UniversityHui-Leen Tan - Pathwest Laboratory MedicineJulie C Pearson - Pathwest Laboratory MedicineGeoffrey W Coombs - Murdoch University
- Publication Details
- Microbial genomics, Vol.9(12), 001172
- Publisher
- Microbiology Society
- Identifiers
- 991005621078807891
- Copyright
- © 2023 The Authors
- Murdoch Affiliation
- Centre for Biosecurity and One Health
- Language
- English
- Resource Type
- Journal article
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