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Journal article
Genotyping-by-sequencing of three mapping populations for identification of candidate genomic regions for resistance to sterility mosaic disease in pigeonpea
Scientific Reports, Vol.7(1), Art. 1813
2017
Abstract
Sterility mosaic disease (SMD) is one of the serious production constraints that may lead to complete yield loss in pigeonpea. Three mapping populations including two recombinant inbred lines and one F2, were used for phenotyping for SMD resistance at two locations in three different years. Genotyping-by-sequencing approach was used for simultaneous identification and genotyping of SNPs on above mentioned populations. In total, 212,464, 89,699 and 64,798 SNPs were identified in ICPL 20096 × ICPL 332 (PRIL_B), ICPL 20097 × ICP 8863 (PRIL_C) and ICP 8863 × ICPL 87119 (F2) respectively. By using high-quality SNPs, genetic maps were developed for PRIL_B (1,101 SNPs; 921.21 cM), PRIL_C (484 SNPs; 798.25 cM) and F2 (996 SNPs; 1,597.30 cM) populations. The average inter marker distance on these maps varied from 0.84 cM to 1.65 cM, which was lowest in all genetic mapping studies in pigeonpea. Composite interval mapping based QTL analysis identified a total of 10 QTLs including three major QTLs across the three populations. The phenotypic variance of the identified QTLs ranged from 3.6 to 34.3%. One candidate genomic region identified on CcLG11 seems to be promising QTL for molecular breeding in developing superior lines with enhanced resistance to SMD.
Details
- Title
- Genotyping-by-sequencing of three mapping populations for identification of candidate genomic regions for resistance to sterility mosaic disease in pigeonpea
- Authors/Creators
- R.K. Saxena (Author/Creator)S.M. Kale (Author/Creator)V. Kumar (Author/Creator)S. Parupali (Author/Creator)S. Joshi (Author/Creator)V. Singh (Author/Creator)V. Garg (Author/Creator)R.R. Das (Author/Creator)M. Sharma (Author/Creator)K.N. Yamini (Author/Creator)A. Ghanta (Author/Creator)A. Rathore (Author/Creator)C.V. Sameer Kumar (Author/Creator)K.B. Saxena (Author/Creator)R.K. Varshney (Author/Creator)
- Publication Details
- Scientific Reports, Vol.7(1), Art. 1813
- Publisher
- Springer Nature
- Identifiers
- 991005543632807891
- Copyright
- © 2017 The Authors.
- Murdoch Affiliation
- State Agricultural Biotechnology Centre; Centre for Crop and Food Innovation; Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.4 Crop Science
- 3.4.96 QTL
- Web Of Science research areas
- Genetics & Heredity
- ESI research areas
- Plant & Animal Science