Genotyping of Cryptosporidium spp. isolated from human stool samples in Switzerland

R. Fretz; P. Svoboda; U. Ryan; R.C.A. Thompson; M. Tanner; A. Baumgartner

doi:10.1017/S0950268803008781

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Genotyping of Cryptosporidium spp. isolated from human stool samples in Switzerland

Journal article

Open access

Peer reviewed

Genotyping of Cryptosporidium spp. isolated from human stool samples in Switzerland

R. Fretz, P. Svoboda, U. Ryan, R.C.A. Thompson, M. Tanner and A. Baumgartner

Epidemiology and Infection, Vol.131(1), pp.663-667

08/2003

DOI: https://doi.org/10.1017/S0950268803008781

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Abstract

In a study to estimate the frequency of Cryptosporidium infections in Switzerland, stool samples from patients found to be positive for Cryptosporidium spp. by modified Ziehl-Neelson staining and fluorescence microscopy were used for genotyping experiments. With 9 of 12 samples, DNA extraction and subsequent genotyping was successful. All Cryptosporidium-isolates belonged to the bovine genotype. In one stool sample, two strains of Cryptosporidium were demonstrated, suggesting a mixed infection. In comparison with reference strains from calves, one of the isolates showed a full sequence identity and the other a similarity of 97.5%. The fact that only bovine genotypes were detected suggests, that cryptosporidiosis must primarily be considered as a zoonotic disease in Switzerland. This is in contrast to other countries, where the human genotype of C. parvum was shown to dominate the epidemiological situation. The results of our study are supported by the previous finding, that two of the analysed strains originated from patients who used to consume raw milk or raw cream, a known risk factor for cryptosporidiosis.

Details

Title: Genotyping of Cryptosporidium spp. isolated from human stool samples in Switzerland
Authors/Creators: R. Fretz (Author/Creator) - Cantonal Laboratory Basel-Landschaft, 4410 Liestal, SwitzerlandSwiss Tropical Institute, 4002 Basel, Switzerland
P. Svoboda (Author/Creator) - Theological Seminary Bienenberg
U. Ryan (Author/Creator) - Murdoch University
R.C.A. Thompson (Author/Creator) - Murdoch University
M. Tanner (Author/Creator) - Swiss Tropical and Public Health Institute
A. Baumgartner (Author/Creator) - Federal Office of Public Health
Publication Details: Epidemiology and Infection, Vol.131(1), pp.663-667
Publisher: Cambridge University Press
Identifiers: 991005541421807891
Copyright: © 2003 Cambridge University Press
Murdoch Affiliation: School of Veterinary and Biomedical Sciences
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 1 Clinical & Life Sciences; 1.246 Diarrheal Diseases; 1.246.985 Cryptosporidium
Web Of Science research areas: Infectious Diseases; Public, Environmental & Occupational Health
ESI research areas: Social Sciences, general