Giardia duodenalis assemblage-specific induction of apoptosis and tight junction disruption in human intestinal epithelial cells: Effects of mixed infections

W.H. Koh; T. Geurden; T. Paget; R. O'Handley; R.F. Steuart; R.C.A. Thompson; A.G. Buret

doi:10.1645/GE-3021.1

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Giardia duodenalis assemblage-specific induction of apoptosis and tight junction disruption in human intestinal epithelial cells: Effects of mixed infections

Journal article

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Giardia duodenalis assemblage-specific induction of apoptosis and tight junction disruption in human intestinal epithelial cells: Effects of mixed infections

W.H. Koh, T. Geurden, T. Paget, R. O'Handley, R.F. Steuart, R.C.A. Thompson and A.G. Buret

Journal of Parasitology, Vol.99(2), pp.353-358

2013

DOI: https://doi.org/10.1645/GE-3021.1

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Abstract

In view of the interest in genotype-specific pathogenesis in Giardia duodenalis, the aim of the present study was to examine the effects of infection with different, or mixed, G. duodenalis assemblages on the integrity of human intestinal epithelia. To that end, human epithelial cells (HCT-8) were cultured and exposed to different G. duodenalis assemblages (A, B, and E) or a combination of these assemblages. Epithelial disruption and apoptosis were evaluated by fluorescent microscopy and apoptotic oligonucleosome quantification. The results indicate that infection with trophozoites disrupts epithelial tight junctions and induces varying degrees of enterocyte apoptosis, depending on the infecting assemblage. All disruptions were caspase-3 dependent and were more pronounced when caused by a non-host specific assemblage. Furthermore, infections by isolates in combination with isolates from another assemblage enhanced the epithelial disruption and apoptosis. Further studies in vitro and in vivo are required to confirm the mechanisms of enhanced pathogenicity of mixed or non-host specific (or both) G. duodenalis infections. Findings in the present study point to the potential pathogenic importance of intra-species polyparasitism in giardiasis.

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Title: Giardia duodenalis assemblage-specific induction of apoptosis and tight junction disruption in human intestinal epithelial cells: Effects of mixed infections
Authors/Creators: W.H. Koh (Author/Creator) - Murdoch University
T. Geurden (Author/Creator) - Ghent University
T. Paget (Author/Creator) - Lehman College
R. O'Handley (Author/Creator)
R.F. Steuart (Author/Creator) - Curtin University
R.C.A. Thompson (Author/Creator) - Murdoch University
A.G. Buret (Author/Creator) - Curtin University
Publication Details: Journal of Parasitology, Vol.99(2), pp.353-358
Publisher: American Society of Parasitologists
Identifiers: 991005541925007891
Copyright: © American Society of Parasitologists 2013
Murdoch Affiliation: School of Veterinary and Biomedical Sciences; School of Veterinary and Life Sciences
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 1 Clinical & Life Sciences; 1.246 Diarrheal Diseases; 1.246.985 Cryptosporidium
Web Of Science research areas: Parasitology
ESI research areas: Microbiology