Global evolutionary dynamics and resistome analysis of Clostridioides difficile ribotype 017

K. Imwattana; P. Putsathit; D.A. Collins; T. Leepattarakit; P. Kiratisin; T.V. Riley; D.R. Knight

doi:10.1099/mgen.0.000792

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Global evolutionary dynamics and resistome analysis of Clostridioides difficile ribotype 017

Journal article

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Global evolutionary dynamics and resistome analysis of Clostridioides difficile ribotype 017

K. Imwattana, P. Putsathit, D.A. Collins, T. Leepattarakit, P. Kiratisin, T.V. Riley and D.R. Knight

Microbial Genomics, Vol.8(3), Art. 000792

2022

DOI: https://doi.org/10.1099/mgen.0.000792

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Abstract

Clostridioides difficile PCR ribotype (RT) 017 ranks among the most successful strains of C. difficile in the world. In the past three decades, it has caused outbreaks on four continents, more than other ‘epidemic’ strains, but our understanding of the genomic epidemiology underpinning the spread of C. difficile RT 017 is limited. Here, we performed high-resolution phylogenomic and Bayesian evolutionary analyses on an updated and more representative dataset of 282 non-clonal C. difficile RT 017 isolates collected worldwide between 1981 and 2019. These analyses place an estimated time of global dissemination between 1953 and 1983 and identified the acquisition of the ermB-positive transposon Tn6194 as a key factor behind global emergence. This coincided with the introduction of clindamycin, a key inciter of C. difficile infection, into clinical practice in the 1960s. Based on the genomic data alone, the origin of C. difficile RT 017 could not be determined; however, geographical data and records of population movement suggest that C. difficile RT 017 had been moving between Asia and Europe since the Middle Ages and was later transported to North America around 1860 (95 % confidence interval: 1622–1954). A focused epidemiological study of 45 clinical C. difficile RT 017 genomes from a cluster in a tertiary hospital in Thailand revealed that the population consisted of two groups of multidrug-resistant (MDR) C. difficile RT 017 and a group of early, non-MDR C. difficile RT 017. The significant genomic diversity within each MDR group suggests that although they were all isolated from hospitalized patients, there was probably a reservoir of C. difficile RT 017 in the community that contributed to the spread of this pathogen.

Details

Title: Global evolutionary dynamics and resistome analysis of Clostridioides difficile ribotype 017
Authors/Creators: K. Imwattana (Author/Creator) - The University of Western Australia
P. Putsathit (Author/Creator) - Edith Cowan University
D.A. Collins (Author/Creator) - Edith Cowan University
T. Leepattarakit (Author/Creator) - Siriraj Hospital
P. Kiratisin (Author/Creator) - Siriraj Hospital
T.V. Riley (Author/Creator) - Pathwest Laboratory Medicine
D.R. Knight (Author/Creator) - Murdoch University
Publication Details: Microbial Genomics, Vol.8(3), Art. 000792
Publisher: Microbiology Society
Identifiers: 991005545019407891
Copyright: © 2022 Imwattana et al.
Murdoch Affiliation: School of Medical, Molecular and Forensic Sciences
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 1 Clinical & Life Sciences; 1.120 Inflammatory Bowel Diseases & Infections; 1.120.1133 Clostridium Infections
Web Of Science research areas: Genetics & Heredity; Microbiology
ESI research areas: Microbiology