Global gene expression analysis of pigeonpea with male sterility conditioned by A 2 cytoplasm

A. Bohra; G. Prasad; A. Rathore; R.K. Saxena; S.J.S. Naik; S. Pareek; R. Jha; L. Pazhamala; D. Datta; G. Pandey; A. Tiwari; A.K. Maurya; K.R. Soren; M. Akram; R.K. Varshney; N.P. Singh

doi:10.1002/tpg2.20132

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Global gene expression analysis of pigeonpea with male sterility conditioned by A 2 cytoplasm

Journal article

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Global gene expression analysis of pigeonpea with male sterility conditioned by A 2 cytoplasm

A. Bohra, G. Prasad, A. Rathore, R.K. Saxena, S.J.S. Naik, S. Pareek, R. Jha, L. Pazhamala, D. Datta, G. Pandey, …

The Plant Genome, Vol.14(3), e20132

2021

DOI: https://doi.org/10.1002/tpg2.20132

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Abstract

Cytoplasmic male sterility(CMS), a maternally inherited trait, provides a promising means to harness yield gains associated with hybrid vigor. In pigeonpea [Cajanus cajan (L.) Huth], nine types of sterility-inducing cytoplasm have been reported, of which A2 and A4 have been successfully deployed in hybrid breeding. Unfortunately, molecular mechanism of the CMS trait is poorly understood because of limited research invested. More recently, an association between a mitochondrial gene (nad7) and A4-CMS has been demonstrated in pigeonpea; however, the mechanism underlying A2-CMS still remains obscure. The current investigation aimed to analyze the differences in A2-CMS line (ICPL 88039A) and its isogenic maintainer line (ICPL 88039B) at transcriptome level using next-generation sequencing. Gene expression profiling uncovered a set of 505 genes that showed altered expression in response to CMS, of which, 412 genes were upregulated while 93 were downregulated in the fertile maintainer line vs. the CMS line. Further, gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and protein–protein interaction (PPI) network analyses revealed association of CMS in pigeonpea with four major pathways: glucose and lipid metabolism, ATP production, pollen development and pollen tube growth, and reactive oxygen species (ROS) scavenging. Patterns of digital gene expression were confirmed by quantitative real-time polymerase chain reaction (qRT-PCR) of six candidate genes. This study elucidates candidate genes and metabolic pathways having potential associations with pollen development and male sterility in pigeonpea A2-CMS. New insights on molecular mechanism of CMS trait in pigeonpea will be helpful to accelerate heterosis utilization for enhancing productivity gains in pigeonpea.

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Title: Global gene expression analysis of pigeonpea with male sterility conditioned by A 2 cytoplasm
Authors/Creators: A. Bohra (Author/Creator) - Indian Institute of Pulses Research
G. Prasad (Author/Creator) - International Crops Research Institute for the Semi-Arid Tropics
A. Rathore (Author/Creator) - International Crops Research Institute for the Semi-Arid Tropics
R.K. Saxena (Author/Creator) - International Crops Research Institute for the Semi-Arid Tropics
S.J.S. Naik (Author/Creator)
S. Pareek (Author/Creator) - Indian Institute of Pulses Research
R. Jha (Author/Creator) - Indian Institute of Pulses Research
L. Pazhamala (Author/Creator) - International Crops Research Institute for the Semi-Arid Tropics
D. Datta (Author/Creator) - Indian Institute of Pulses Research
G. Pandey (Author/Creator) - Indian Institute of Pulses Research
A. Tiwari (Author/Creator) - Indian Institute of Pulses Research
A.K. Maurya (Author/Creator) - Indian Institute of Pulses Research
K.R. Soren (Author/Creator) - Indian Institute of Pulses Research
M. Akram (Author/Creator) - Indian Institute of Pulses Research
R.K. Varshney (Author/Creator) - International Crops Research Institute for the Semi-Arid Tropics
N.P. Singh (Author/Creator) - Indian Institute of Pulses Research
Publication Details: The Plant Genome, Vol.14(3), e20132
Publisher: Wiley Periodicals LLC on behalf of Crop Science Society of America
Identifiers: 991005540781307891
Copyright: © 2021 The Authors.
Murdoch Affiliation: Centre for Crop and Food Innovation; State Agricultural Biotechnology Centre; Food Futures Institute
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.4 Crop Science; 3.4.1922 Alternative Oxidase
Web Of Science research areas: Genetics & Heredity; Plant Sciences
ESI research areas: Plant & Animal Science