Green fluorescent protein-based biosensor to detect and quantify stress responses induced by DNA-degrading colicins

S. Abraham; J. Chin; H.J.M. Brouwers; B. Turner; R. Zhang; T.A. Chapman

doi:10.1128/AEM.00534-11

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Green fluorescent protein-based biosensor to detect and quantify stress responses induced by DNA-degrading colicins

Journal article

Open access

Peer reviewed

Green fluorescent protein-based biosensor to detect and quantify stress responses induced by DNA-degrading colicins

S. Abraham, J. Chin, H.J.M. Brouwers, B. Turner, R. Zhang and T.A. Chapman

Applied and Environmental Microbiology, Vol.77(18), pp.6691-6693

2011

DOI: https://doi.org/10.1128/AEM.00534-11

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Abstract

Here we report the development of a whole-cell biosensor to detect and quantify the induction of the SOS response activated by DNA-degrading colicins. This biosensor utilizes the SOS-responsive cda promoter to regulate the expression of green fluorescent protein. The biosensor assay revealed induction of stress for all DNA-degrading reference colicins (E2, E7, and E8).

Details

Title: Green fluorescent protein-based biosensor to detect and quantify stress responses induced by DNA-degrading colicins
Authors/Creators: S. Abraham (Author/Creator) - New South Wales Department of Primary Industries
J. Chin (Author/Creator) - New South Wales Department of Primary Industries
H.J.M. Brouwers (Author/Creator) - New South Wales Department of Primary Industries
B. Turner (Author/Creator) - New South Wales Department of Primary Industries
R. Zhang (Author/Creator) - University of Wollongong
T.A. Chapman (Author/Creator) - New South Wales Department of Primary Industries
Publication Details: Applied and Environmental Microbiology, Vol.77(18), pp.6691-6693
Publisher: American Society for Microbiology
Identifiers: 991005542392307891
Copyright: © 2011, American Society for Microbiology.
Murdoch Affiliation: Murdoch University
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration
Citation topics: 1 Clinical & Life Sciences; 1.42 Bacteriology; 1.42.839 Outer Membrane
Web Of Science research areas: Biotechnology & Applied Microbiology; Microbiology
ESI research areas: Biology & Biochemistry