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Growth characteristics, nitrogen uptake and enzyme activities of the nitrate-utilising ectomycorrhizal Scleroderma verrucosum
Journal article   Open access   Peer reviewed

Growth characteristics, nitrogen uptake and enzyme activities of the nitrate-utilising ectomycorrhizal Scleroderma verrucosum

D. Prima Putra, A. Berredjem, M. Chalot, B. Dell and B. Botton
Mycological Research, Vol.103(8), pp.997-1002
1999
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Abstract

The growth characteristics and uptake capacities for NO3- and NH4+, as well as activities of nitrogen assimilating-enzymes have been determined in Scleroderma verrucosum. Biomass, grown on agar or liquid MMN media, was higher on nitrate. When S. verrucosum was grown in the presence of equal amounts of ammonium and nitrate, uptake rate for NH4+ was considerably higher than that of NO3-. In addition, it was found that a limitation in ammonium concentration was a prerequisite for nitrate uptake. Nitrate reductase was stimulated when the fungus was transferred from ammonium to nitrate containing media, indicating that the enzyme was inducible by nitrate and repressed by ammonium. Glutamine synthetase and NAD-glutamate synthase activities were clearly detected in S. verrucosum, while the NADP-glutamate dehydrogenase was almost undetectable. This is consistent with the view that ammonium assimilation occurs through the GS/GOGAT cycle in S. verrucosum.

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Collaboration types
Domestic collaboration
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3 Agriculture, Environment & Ecology
3.97 Plant Pathology
3.97.488 Mycorrhizal Symbiosis
Web Of Science research areas
Mycology
ESI research areas
Plant & Animal Science
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