HCV RNA traffic and association with NS5A in living cells

G.N. Fiches; N.S. Eyre; A.L. Aloia; K. Van Der Hoek; B. Betz-Stablein; F. Luciani; A. Chopra; M.R. Beard

doi:10.1016/j.virol.2016.02.016

Back

HCV RNA traffic and association with NS5A in living cells

Journal article

Peer reviewed

HCV RNA traffic and association with NS5A in living cells

G.N. Fiches, N.S. Eyre, A.L. Aloia, K. Van Der Hoek, B. Betz-Stablein, F. Luciani, A. Chopra and M.R. Beard

Virology, Vol.493, pp.60-74

2016

DOI: https://doi.org/10.1016/j.virol.2016.02.016

Files and links (1)

url

Free to Read *No subscription requiredView

Abstract

The spatiotemporal dynamics of Hepatitis C Virus (HCV) RNA localisation are poorly understood. To address this we engineered HCV genomes harbouring MS2 bacteriophage RNA stem-loops within the 3′-untranslated region to allow tracking of HCV RNA via specific interaction with a MS2-Coat-mCherry fusion protein. Despite the impact of these insertions on viral fitness, live imaging revealed that replication of tagged-HCV genomes induced specific redistribution of the mCherry-tagged-MS2-Coat protein to motile and static foci. Further analysis showed that HCV RNA was associated with NS5A in both static and motile structures while a subset of motile NS5A structures was devoid of HCV RNA. Further investigation of viral RNA traffic with respect to lipid droplets (LDs) revealed HCV RNA-positive structures in close association with LDs. These studies provide new insights into the dynamics of HCV RNA traffic with NS5A and LDs and provide a platform for future investigations of HCV replication and assembly.

Details

Title: HCV RNA traffic and association with NS5A in living cells
Authors/Creators: G.N. Fiches (Author/Creator) - Centre for Cancer Biology
N.S. Eyre (Author/Creator) - Centre for Cancer Biology
A.L. Aloia (Author/Creator) - Centre for Cancer Biology
K. Van Der Hoek (Author/Creator) - Centre for Cancer Biology
B. Betz-Stablein (Author/Creator) - UNSW Sydney
F. Luciani (Author/Creator) - UNSW Sydney
A. Chopra (Author/Creator) - Murdoch University
M.R. Beard (Author/Creator) - Centre for Cancer Biology
Publication Details: Virology, Vol.493, pp.60-74
Publisher: Elsevier
Identifiers: 991005543211807891
Copyright: © 2016 Elsevier Inc.
Murdoch Affiliation: Institute for Immunology and Infectious Diseases
Language: English
Resource Type: Journal article

UN Sustainable Development Goals (SDGs)

This output has contributed to the advancement of the following goals:

Source: InCites

Metrics

32 Record Views

10 Times Cited - Web of Science

InCites Highlights

These are selected metrics from InCites Benchmarking & Analytics tool, related to this output

Collaboration types: Domestic collaboration
Citation topics: 1 Clinical & Life Sciences; 1.125 Hepatitis; 1.125.83 HCV
Web Of Science research areas: Virology
ESI research areas: Microbiology