Files and links (1)
Open Access CC BY-NC V4.0
Journal article
HDAC2-mediated H3K27ac Governs ZFP42 transcription and autophagy in granulosa cells of pigs
Life sciences (1973), Vol.401, 124527
2026
PMID: 42264139
Abstract
Aims
Histone deacetylation regulates mammalian follicular development via ovarian granulosa cells (GCs) autophagy, but the underlying mechanisms remain largely unknown. This study aimed to elucidate the epigenetic role of HDAC2 in GC autophagy and follicular development through ZFP42.
Materials and methods
HDAC2 knockdown combined with autophagy detection were performed to identify the effect of HDAC2 on autophagy and ZFP42 in GCs. Subsequently, chromatin accessibility assay and CRISPR/dCas9-P300 epigenetic editing were used to explore the molecular mechanism that HDAC2 regulates the expression of ZFP42. Finally, In vitro and in vivo methods were applied to evaluate the functional effects of ZFP42 on autophagy and follicular development.
Key findings
Knockdown of HDAC2 Induces autophagy in GCs. ZFP42 protein level dynamically decreased during follicular development. Chromatin accessibility assay and CRISPR/dCas9-P300 editing revealed that HDAC2 dynamically altered H3K27ac occupancy at the promoter of ZFP42 to activating transcriptional activation of ZFP42. Functionally, ZFP42 protein interacted with the autophagic marker Beclin1 to modulate autophagy. Moreover, ZFP42 promoted GC autophagy and apoptosis to decrease serum estradiol (E2) levels and block follicular development in vivo.
Significance
These results provided mechanistic insights into ovarian follicle development, and proposed ZFP42 as a potential therapeutic target for autophagy-related reproductive disorders.
Details
- Title
- HDAC2-mediated H3K27ac Governs ZFP42 transcription and autophagy in granulosa cells of pigs
- Authors/Creators
- Weili Liao - Guangdong Academy of Agricultural SciencesNian Li - South China Agricultural UniversityYao Jiang - Agricultural Genomics Institute at ShenzhenEnyuan Huang - South China Agricultural UniversityKe Mao - Chinese Academy of SciencesBin Ma - Murdoch University, School of Medical, Molecular and Forensic SciencesJianguo Zhao - Guangdong Academy of Agricultural SciencesXiaolong Yuan - South China Agricultural University
- Publication Details
- Life sciences (1973), Vol.401, 124527
- Publisher
- Elsevier Inc.
- Identifiers
- 991005898162807891
- Copyright
- © 2026 The Authors.
- Murdoch Affiliation
- Centre for Healthy Ageing; School of Medical, Molecular and Forensic Sciences
- Language
- English
- Resource Type
- Journal article
Metrics
1 Record Views