Journal article
HLA-B*5701 typing: evaluation of an allele-specific polymerase chain reaction melting assay
Tissue Antigens, Vol.70(1), pp.58-61
07/2007
Abstract
Inheritance of HLA-B*5701 is a strong predictor of a hypersensitivity reaction to the anti-HIV drug abacavir. The identification of susceptible individuals prior to the institution of abacavir therapy is therefore of clinical importance and has generated demand for a simple and rapid diagnostic test for carriage of HLA-B*5701. In this study, we describe the development of such a method based on allele-specific polymerase chain reaction (AS-PCR) and melting curve analysis. Ninety-six patient samples including 36 HLA-B*5701-positive samples and 60 HLA-B*5701-negative samples were analysed. Compared with sequence-based typing, this method had 100% sensitivity and specificity for the HLA-B*5701 allele. In conclusion, the AS-PCR/melting curve approach minimises post-polymerase chain reaction handling processing and provides an attractive alternative to currently described AS-PCR methods.
Details
- Title
- HLA-B*5701 typing: evaluation of an allele-specific polymerase chain reaction melting assay
- Authors/Creators
- E. Hammond (Author/Creator)C. Mamotte (Author/Creator)D. Nolan (Author/Creator)S. Mallal (Author/Creator)
- Publication Details
- Tissue Antigens, Vol.70(1), pp.58-61
- Publisher
- Blackwell Publishing
- Identifiers
- 991005544929407891
- Copyright
- © 2007 Blackwell Munksgaard.
- Murdoch Affiliation
- Centre for Clinical Immunology and Biomedical Statistics
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites
Metrics
50 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.265 Dermatology - Skin Allergies
- 1.265.1140 Drug Hypersensitivity
- Web Of Science research areas
- Cell Biology
- Immunology
- Pathology
- ESI research areas
- Molecular Biology & Genetics