Journal article
Haplotyping the human leukocyte antigen system from single chromosomes
Scientific Reports, Vol.6
2016
Abstract
We describe a method for determining the parental HLA haplotypes of a single individual without recourse to conventional segregation genetics. Blood samples were cultured to identify and sort chromosome 6 by bivariate flow cytometry. Single chromosome 6 amplification products were confirmed with a single nucleotide polymorphism (SNP) array and verified by deep sequencing to enable assignment of both alleles at the HLA loci, defining the two haplotypes. This study exemplifies a rapid and efficient method of haplotyping that can be applied to any chromosome pair, or indeed all chromosome pairs, using a single sorting operation. The method represents a cost-effective approach to complete phasing of SNPs, which will facilitate a deeper understanding of the links between SNPs, gene regulation and protein function.
Details
- Title
- Haplotyping the human leukocyte antigen system from single chromosomes
- Authors/Creators
- N.M. Murphy (Author/Creator) - Monash UniversityM. Burton (Author/Creator) - Murdoch Children's Research InstituteD.R. Powell (Author/Creator) - Monash UniversityF.J. Rossello (Author/Creator) - Australian Regenerative Medicine InstituteD. Cooper (Author/Creator) - Murdoch UniversityA. Chopra (Author/Creator) - Murdoch UniversityM.J. Hsieh (Author/Creator) - Monash UniversityD.C. Sayer (Author/Creator) - Genomics (United Kingdom)L. Gordon (Author/Creator) - Walter and Eliza Hall Institute of Medical ResearchM.D. Pertile (Author/Creator) - The University of MelbourneB.D. Tait (Author/Creator) - Australian Red Cross LifebloodH.R. Irving (Author/Creator) - Monash UniversityC.W. Pouton (Author/Creator) - Monash University
- Publication Details
- Scientific Reports, Vol.6
- Publisher
- Springer Nature
- Identifiers
- 991005543714207891
- Copyright
- © 2016 Macmillan Publishers Limited
- Murdoch Affiliation
- Institute for Immunology and Infectious Diseases
- Language
- English
- Resource Type
- Journal article
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Source: InCites
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- Collaboration types
- Domestic collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.6 Immunology
- 1.6.607 MHC Diversity
- Web Of Science research areas
- Genetics & Heredity
- ESI research areas
- Molecular Biology & Genetics