Journal article
Helicobacter pylori adaptation in vivoin response to a high salt diet
Infection and Immunity
2015
Abstract
Helicobacter pylori exhibits a high level of intraspecies genetic diversity. In this study, we investigated whether the diversification of H. pylori is influenced by the composition of the diet. Specifically, we investigated the effect of a high salt diet (a known risk factor for gastric adenocarcinoma) on H. pylori diversification within a host. We analyzed H. pylori strains isolated from Mongolian gerbils fed either a high salt diet or a regular diet for four months, using proteomic and whole genome sequencing methods. Compared to the input strain and output strains from animals fed a regular diet, the output strains from animals fed a high salt diet produced higher levels of proteins involved in iron acquisition and oxidative stress resistance. Several of these changes were attributable to a non-synonymous mutation in fur (fur-R88H). Further experiments indicated that this mutation conferred increased resistance to high salt conditions and oxidative stress. We propose a model in which a high salt diet leads to high levels of gastric inflammation and associated oxidative stress in H. pylori-infected animals, and that these conditions along with the high intraluminal concentrations of sodium chloride lead to selection of H. pylori strains that are most fit for growth in this environment.
Details
- Title
- Helicobacter pylori adaptation in vivoin response to a high salt diet
- Authors/Creators
- J.T. Loh (Author/Creator) - Vanderbilt UniversityJ.A. Gaddy (Author/Creator) - Vanderbilt UniversityH.M.S. Algood (Author/Creator) - Vanderbilt UniversityS. Gaudieri (Author/Creator) - Vanderbilt UniversityS. Mallal (Author/Creator) - Vanderbilt UniversityT.L. Cover (Author/Creator) - Vanderbilt University
- Publication Details
- Infection and Immunity
- Publisher
- American Society for Microbiology
- Identifiers
- 991005544887907891
- Murdoch Affiliation
- Institute for Immunology and Infectious Diseases
- Language
- English
- Resource Type
- Journal article
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Source: InCites
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.95 Gastrointestinal & Esophageal Diseases
- 1.95.387 Helicobacter Pylori
- Web Of Science research areas
- Immunology
- Infectious Diseases
- ESI research areas
- Immunology