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Journal article
Hematology and toll-like receptors 2 and 4 and lipopolysaccharide-induced tumor necrosis factor-alpha factor gene expression in peripheral blood mononuclear cells of Thai indigenous chickens
Veterinary World, Vol.15(12), pp.2795-2799
2022
PMID: 36718338
Abstract
Background and Aim: Toll-like receptors (TLRs) play crucial roles in the early phase of infection in the innate immune response against bacteria, viruses, fungi, and parasites. Lipopolysaccharide-induced tumor necrosis factor-alpha factor (LITAF) is an essential transcription factor that regulates the immune system, apoptosis, and inflammatory cytokines. This study aimed to determine the hematological profile reflecting the immune response related to TLR2 and TLR4 and LITAF gene expression in Thai indigenous chickens. Materials and Methods: Blood samples (2 mL) were randomly obtained from three chicken breeds (black-boned chicken, Fah Luang chicken, and Pradu Hang Dam chicken) at 16 weeks of age (n = 5 per breed). The hematological profile and mRNA expression within the peripheral blood mononuclear cells (PBMCs) were determined by hematological analysis and quantitative real-time polymerase chain reaction (qRT-PCR).Results: The hematological profile differed significantly in terms of red blood cells (RBCs), hemoglobin, and white blood cells (WBCs) (p < 0.05). Black-boned chicken and Fah Luang chicken had lower RBC levels than Pradu Hang Dam chicken. Fah Luang chicken had lower hemoglobin than Pradu Hang Dam chicken. However, Fah Luang chicken had higher WBC levels than Pradu Hang Dam chicken. Hematocrit, heterophils, basophils, eosinophils, lymphocytes, and monocytes did not differ significantly among the groups (p > 0.05). According to qRT-PCR, the expression of the TLR2 gene did not differ significantly among the groups (p > 0.05), while TLR4 and LITAF gene expression did (p < 0.05). Toll-like receptor 4 and LITAF genes were most highly expressed in Fah Luang chicken.Conclusion: The PBMCs of Thai indigenous chickens showed evidence of TLR4 and LITAF gene expression, with higher expression levels observed in Fah Luang chicken. From this preliminary study, it is concluded that TLR4 and LITAF genes might play roles in the main immune system response in Thai indigenous chickens.
Details
- Title
- Hematology and toll-like receptors 2 and 4 and lipopolysaccharide-induced tumor necrosis factor-alpha factor gene expression in peripheral blood mononuclear cells of Thai indigenous chickens
- Authors/Creators
- Chananphat Tantikositruj - Kasetsart UniversityAsep Gunawan - IPB UniversityMuhammad Jasim Uddin - Murdoch University, Centre for Animal Production and HealthWirawan Nuchchanart - Kasetsart UniversityChaiwat Boonkaewwan - Walailak UniversityWatchara Laenoi - University of PhayaoAutchara Kayan - Kasetsart University
- Publication Details
- Veterinary World, Vol.15(12), pp.2795-2799
- Publisher
- Veterinary World
- Number of pages
- 5
- Grant note
- RUP2.2/Con-CASAF PM 09 / Kasetsart University Reinventing University Program 2021 Office of the Ministry of Higher Education, Science, Research and Innovation
- Identifiers
- 991005585961807891
- Copyright
- © 2022 Tantikositruj, et al.
- Murdoch Affiliation
- School of Veterinary Medicine
- Language
- English
- Resource Type
- Journal article
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