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Journal article
Hepatic transcriptome analysis reveals genes, polymorphisms, and molecules related to Lamb tenderness
Animals (Basel), Vol.13(4), Art. 674
2023
PMID: 36830461
Abstract
Simple Summary Tenderness influences repurchase decisions of sheep meat because it is a significant factor contributing to eating satisfaction and consumer acceptance. This study analyzed the transcriptome of five high- and five low-lamb tenderness samples. The result showed potential candidate hepatic genes and polymorphisms affecting lamb tenderness. These potential candidate genes and genetic markers could be used in lamb tenderness selection programs. Tenderness is a key meat quality trait that determines the public acceptance of lamb consumption, so genetic improvement toward lamb with higher tenderness is pivotal for a sustainable sheep industry. However, unravelling the genomics controlling the tenderness is the first step. Therefore, this study aimed to identify the transcriptome signatures and polymorphisms related to divergent lamb tenderness using RNA deep sequencing. Since the molecules and enzymes that control muscle growth and tenderness are metabolized and synthesized in the liver, hepatic tissues of ten sheep with divergent phenotypes: five high- and five low-lamb tenderness samples were applied for deep sequencing. Sequence analysis identified the number of reads ranged from 21.37 to 25.37 million bases with a mean value of 22.90 million bases. In total, 328 genes are detected as differentially expressed (DEGs) including 110 and 218 genes that were up- and down-regulated, respectively. Pathway analysis showed steroid hormone biosynthesis as the dominant pathway behind the lamb tenderness. Gene expression analysis identified the top high (such as TP53INP1, CYP2E1, HSD17B13, ADH1C, and LPIN1) and low (such as ANGPTL2, IGFBP7, FABP5, OLFML3, and THOC5) expressed candidate genes. Polymorphism and association analysis revealed that mutation in OLFML3, ANGPTL2, and THOC5 genes could be potential candidate markers for tenderness in sheep. The genes and pathways identified in this study cause variation in tenderness, thus could be potential genetic markers to improve meat quality in sheep. However, further validation is needed to confirm the effect of these markers in different sheep populations so that these could be used in a selection program for lamb with high tenderness.
Details
- Title
- Hepatic transcriptome analysis reveals genes, polymorphisms, and molecules related to Lamb tenderness
- Authors/Creators
- Kasita Listyarini - IPB UniversityCece Sumantri - IPB UniversitySri Rahayu - IPB Univ, Fac Anim Sci, Dept Anim Prod & Technol, Bogor 16680, IndonesiaMd. Aminul Islam - Bangladesh Agricultural UniversitySyeda Hasina Akter - Bangladesh Agricultural UniversityMuhammad Jasim Uddin - Murdoch University, Centre for Animal Production and HealthAsep Gunawan - IPB University
- Publication Details
- Animals (Basel), Vol.13(4), Art. 674
- Publisher
- MDPI
- Number of pages
- 19
- Grant note
- 001/E5/PG.02.00PT/2022 / RISTEKDIKTI; Ministry of Research and Technology of the Republic of Indonesia (RISTEK) IPB University
- Identifiers
- 991005565369107891
- Copyright
- © 2023 by the authors.
- Murdoch Affiliation
- Centre for Biosecurity and One Health; School of Veterinary Medicine
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.51 Dairy & Animal Sciences
- 3.51.206 Meat Quality
- Web Of Science research areas
- Agriculture, Dairy & Animal Science
- Veterinary Sciences
- Zoology
- ESI research areas
- Plant & Animal Science