Journal article
High-Throughput Microbore UPLC-MS Metabolic Phenotyping of Urine for Large-Scale Epidemiology Studies
Journal of proteome research, Vol.14(6), pp.2714-2721
2015
PMID: 25917015
Abstract
A new generation of metabolic phenotyping centers are being created to meet the increasing demands of personalized healthcare, and this has resulted in a major requirement for economical, high-throughput metabonomic analysis by liquid chromatography–mass spectrometry (LC–MS). Meeting these new demands represents an emerging bioanalytical problem that must be solved if metabolic phenotyping is to be successfully applied to large clinical and epidemiological sample sets. Ultraperformance (UP)LC–MS-based metabolic phenotyping, based on 2.1 mm i.d. LC columns, enables comprehensive metabolic phenotyping but, when employed for the analysis of thousands of samples, results in high solvent usage. The use of UPLC–MS employing 1 mm i.d. columns for metabolic phenotyping rather than the conventional 2.1 mm i.d. methodology shows that the resulting optimized microbore method provided equivalent or superior performance in terms of peak capacity, sensitivity, and robustness. On average, we also observed, when using the microbore scale separation, an increase in response of 2-3 fold over that obtained with the standard 2.1 mm scale method. When applied to the analysis of human urine, the 1 mm scale method showed no decline in performance over the course of 1000 analyses, illustrating that microbore UPLC–MS represents a viable alternative to conventional 2.1 mm i.d. formats for routine large-scale metabolic profiling studies while also resulting in a 75% reduction in solvent usage. The modest increase in sensitivity provided by this methodology also offers the potential to either reduce sample consumption or increase the number of metabolite features detected with confidence due to the increased signal-to-noise ratios obtained. Implementation of this miniaturized UPLC–MS method of metabolic phenotyping results in clear analytical, economic, and environmental benefits for large-scale metabolic profiling studies with similar or improved analytical performance compared to conventional UPLC–MS.
Details
- Title
- High-Throughput Microbore UPLC-MS Metabolic Phenotyping of Urine for Large-Scale Epidemiology Studies
- Authors/Creators
- Nicola Gray - College Station Medical CenterMatthew R. Lewis - Hammersmith HospitalRobert S. Plumb - College Station Medical CenterIan D. Wilson - College Station Medical CenterJeremy K. Nichoson - College Station Medical Center
- Publication Details
- Journal of proteome research, Vol.14(6), pp.2714-2721
- Publisher
- Amer Chemical Soc
- Number of pages
- 8
- Grant note
- Bruker Biospin MC_PC_12025 / National Institute of Health Research (England); National Institute for Health Research (NIHR) MC_PC_12025 / UK Medical Research Council; UK Research & Innovation (UKRI); Medical Research Council UK (MRC) Metabometrix Ltd Imperial College MC_PC_12025 / Medical Research Council; UK Research & Innovation (UKRI); Medical Research Council UK (MRC); European Commission Waters Corporation MC_PC_12025 / MRC; UK Research & Innovation (UKRI); Medical Research Council UK (MRC)
- Identifiers
- 991005600060307891
- Copyright
- © 2015 American Chemical Society
- Murdoch Affiliation
- Centre for Computational and Systems Medicine
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 2 Chemistry
- 2.211 Mass Spectrometry
- 2.211.990 Metabolomics
- Web Of Science research areas
- Biochemical Research Methods
- ESI research areas
- Biology & Biochemistry