Journal article
High-density SNP map facilitates fine mapping of QTLs and candidate genes discovery for Aspergillus flavus resistance in peanut (Arachis hypogaea)
Theoretical and Applied Genetics, Vol.133(7), pp.2239-2257
2020
Abstract
Aflatoxin contamination in peanuts caused by Aspergillus flavus is a serious food safety issue for human health around the world. Host plant resistance to fungal infection and reduction in aflatoxin are crucial for mitigating this problem. Identification of the resistance-linked markers can be used in marker-assisted breeding for varietal development. Here we report construction of two high-density genetic linkage maps with 1975 SNP loci and 5022 SNP loci, respectively. Two consistent quantitative trait loci (QTL) were identified as qRAF-3-1 and qRAF-14-1, which located on chromosomes A03 and B04, respectively. QTL qRAF-3-1 was mapped within 1.67 cM and had more than 19% phenotypic variance explained (PVE), while qRAF-14-1 was located within 1.34 cM with 5.15% PVE. While comparing with the reference genome, the mapped QTLs, qRAF-3-1 and qRAF-14-1, were located within a physical distance of 1.44 Megabase pair (Mbp) and 2.22 Mbp, harboring 67 and 137 genes, respectively. Among the identified candidate genes, six genes with the same function were found within both QTLs regions. In addition, putative disease resistance RPP13-like protein 1 (RPP13), lipoxygenase (Lox), WRKY transcription factor (WRKY) and cytochrome P450 71B34 genes were also identified. Using microarray analysis, genes responded to A. flavus infection included coding for RPP13, pentatricopeptide repeat-containing-like protein, and Lox which may be possible candidate genes for resistance to A. flavus. The QTLs and candidate genes will further facilitate marker development and validation of genes for deployment in the molecular breeding programs against A. flavus in peanuts.
Details
- Title
- High-density SNP map facilitates fine mapping of QTLs and candidate genes discovery for Aspergillus flavus resistance in peanut (Arachis hypogaea)
- Authors/Creators
- S.A. Khan (Author/Creator)H. Chen (Author/Creator)Y. Deng (Author/Creator) - Fujian Agriculture and Forestry UniversityY. Chen (Author/Creator) - Fujian Agriculture and Forestry UniversityC. Zhang (Author/Creator)T. Cai (Author/Creator) - Fujian Agriculture and Forestry UniversityN. Ali (Author/Creator) - Fujian Agriculture and Forestry UniversityG. Mamadou (Author/Creator) - Fujian Agriculture and Forestry UniversityD. Xie (Author/Creator) - Fujian Agriculture and Forestry UniversityB. Guo (Author/Creator) - Agricultural Research ServiceR.K. Varshney (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsW. Zhuang (Author/Creator) - Fujian Agriculture and Forestry University
- Publication Details
- Theoretical and Applied Genetics, Vol.133(7), pp.2239-2257
- Publisher
- Springer Verlag
- Identifiers
- 991005544525807891
- Copyright
- © 2021 Springer Nature Switzerland AG.
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.4 Crop Science
- 3.4.96 QTL
- Web Of Science research areas
- Agronomy
- Genetics & Heredity
- Horticulture
- Plant Sciences
- ESI research areas
- Agricultural Sciences