High fat diet causes distinct aberrations in the testicular proteome

S Jarvis; L A Gethings; L Samanta; S M A Pedroni; D J Withers; N Gray; R S Plumb; R M L Winston; C Williamson; C L Bevan

doi:10.1038/s41366-020-0595-6

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High fat diet causes distinct aberrations in the testicular proteome

Journal article

Open access

Peer reviewed

High fat diet causes distinct aberrations in the testicular proteome

S Jarvis, L A Gethings, L Samanta, S M A Pedroni, D J Withers, N Gray, R S Plumb, R M L Winston, C Williamson and C L Bevan

International journal of obesity (2005), Vol.44(9), pp.1958-1969

2020

DOI: https://doi.org/10.1038/s41366-020-0595-6

PMCID: 7445115

PMID: 32678325

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Abstract

Animals

Diet, High-Fat - adverse effects

Dietary Fats - pharmacology

Humans

Male

Mice

Obesity - metabolism

Proteome - drug effects

Testis - drug effects

Testis - pathology

Diet has important effects on normal physiology and the potential deleterious effects of high fat diets and obesity on male reproductive health are being increasingly described. We conducted a histological review of the effects of chronic high fat (HF) diet (using a mouse model fed a 45% fat diet for 21 weeks) with a discovery proteomic study to assess for changes in the abundance of proteins in the testis. Mice on a HF diet became obese and developed glucose intolerance. Using mass spectrometry, we identify 102 proteins affected in the testis of obese mice. These included structural proteins important for the blood testis barrier (filamin A, FLNA), proteins involved in oxidative stress responses (spermatogenesis associated 20, SPATA-20) and lipid homoeostasis (sterol regulatory element-binding protein 2, SREBP2 and apolipoprotein A1, APOA1). In addition, an important regulator protein paraspeckle component 1, PSPC-1, which interacts with the androgen receptor was significantly downregulated. Proteomic data was validated using both Western blotting and immunostaining which confirmed and localised protein expression in both mouse and human testis using biopsy specimens. This study focused mainly on the abnormalities that occurred at the protein level and as a result, we have identified several candidate proteins and conducted pathway analysis around the effects of HF diet on the testis providing novel insights not previously described. Some of the identified targets could be targeted therapeutically and future work is directed in this area.

Details

Title: High fat diet causes distinct aberrations in the testicular proteome
Authors/Creators: S Jarvis - Hammersmith Hospital
L A Gethings - Waters (United Kingdom)
L Samanta - Cleveland Clinic
S M A Pedroni - MRC London Institute of Medical Sciences
D J Withers - MRC London Institute of Medical Sciences
N Gray - Division of Computational and Systems Medicine, Sir Alexander Fleming Building South Kensington, London, SW7 2AZ, UK
R S Plumb - Waters (United States)
R M L Winston - Hammersmith Hospital
C Williamson - King's College London
C L Bevan - Hammersmith Hospital
Publication Details: International journal of obesity (2005), Vol.44(9), pp.1958-1969
Publisher: Springer Nature
Grant note: Wellcome Trust MC_U120097114 / Medical Research Council
Identifiers: 991005600058807891
Murdoch Affiliation: Centre for Computational and Systems Medicine
Language: English
Resource Type: Journal article

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Source: InCites

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Collaboration types: Industry collaboration; Domestic collaboration; International collaboration
Citation topics: 1 Clinical & Life Sciences; 1.81 Reproductive Biology; 1.81.176 Male Fertility
Web Of Science research areas: Endocrinology & Metabolism; Nutrition & Dietetics
ESI research areas: Clinical Medicine