Journal article
High-mass-resolution MALDI mass spectrometry imaging reveals detailed spatial distribution of metabolites and lipids in roots of barley seedlings in response to salinity stress
Metabolomics, Vol.14, Article number: 63
2018
Abstract
Introduction
Mass spectrometry imaging (MSI) is a technology that enables the visualization of the spatial distribution of hundreds to thousands of metabolites in the same tissue section simultaneously. Roots are below-ground plant organs that anchor plants to the soil, take up water and nutrients, and sense and respond to external stresses. Physiological responses to salinity are multifaceted and have predominantly been studied using whole plant tissues that cannot resolve plant salinity responses spatially.
Objectives
This study aimed to use a comprehensive approach to study the spatial distribution and profiles of metabolites, and to quantify the changes in the elemental content in young developing barley seminal roots before and after salinity stress.
Methods
Here, we used a combination of liquid chromatography–mass spectrometry (LC–MS), inductively coupled plasma mass spectrometry (ICP–MS), and matrix-assisted laser desorption/ionization (MALDI–MSI) platforms to profile and analyze the spatial distribution of ions, metabolites and lipids across three anatomically different barley root zones before and after a short-term salinity stress (150 mM NaCl).
Results
We localized, visualized and discriminated compounds in fine detail along longitudinal root sections and compared ion, metabolite, and lipid composition before and after salt stress. Large changes in the phosphatidylcholine (PC) profiles were observed as a response to salt stress with PC 34:n showing an overall reduction in salt treated roots. ICP–MS analysis quantified changes in the elemental content of roots with increases of Na+ and decreases of K+ content.
Conclusion
Our results established the suitability of combining three mass spectrometry platforms to analyze and map ionic and metabolic responses to salinity stress in plant roots and to elucidate tolerance mechanisms in response to abiotic stress, such as salinity stress.
Details
- Title
- High-mass-resolution MALDI mass spectrometry imaging reveals detailed spatial distribution of metabolites and lipids in roots of barley seedlings in response to salinity stress
- Authors/Creators
- L.D. Sarabia (Author/Creator) - The University of MelbourneB.A. Boughton (Author/Creator) - The University of MelbourneT.W.T. Rupasinghe (Author/Creator)A.M.L. van de Meene (Author/Creator) - The University of MelbourneD.L. Callahan (Author/Creator) - Deakin UniversityC.B. Hill (Author/Creator) - Murdoch UniversityU. Roessner (Author/Creator) - The University of Melbourne
- Publication Details
- Metabolomics, Vol.14, Article number: 63
- Publisher
- Springer
- Identifiers
- 991005545271807891
- Copyright
- © The Author(s) 2018
- Murdoch Affiliation
- Western Barley Genetics Alliance
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- Citation topics
- 2 Chemistry
- 2.211 Mass Spectrometry
- 2.211.304 Mass Spectrometry Advances
- Web Of Science research areas
- Endocrinology & Metabolism
- ESI research areas
- Biology & Biochemistry