High prevalence of toxigenic and nontoxigenic clostridium difficile strains in Malaysia

T.V. Riley; D.A. Collins; R. Karunakaran; M.A. Kahar; A. Adnan; S.A. Hassan; N.H. Zainul; F.R.M. Rustam; Z.A. Wahab; R. Ramli; Y.Y. Lee; H. Hassan; A.B. Onderdonk

doi:10.1128/JCM.00170-18

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High prevalence of toxigenic and nontoxigenic clostridium difficile strains in Malaysia

Journal article

Peer reviewed

High prevalence of toxigenic and nontoxigenic clostridium difficile strains in Malaysia

T.V. Riley, D.A. Collins, R. Karunakaran, M.A. Kahar, A. Adnan, S.A. Hassan, N.H. Zainul, F.R.M. Rustam, Z.A. Wahab, R. Ramli, …

Journal of Clinical Microbiology, Vol.56(6)

2018

DOI: https://doi.org/10.1128/JCM.00170-18

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Abstract

Accumulating evidence shows a high prevalence of Clostridium difficile in Southeast Asia associated with a range of clinical presentations. However, severe infections are rarely reported. We investigated C. difficile infection (CDI) across four hospitals in Kuala Lumpur and Kota Bharu, Malaysia. Enzyme immunoassays for glutamate dehydrogenase (GDH) and toxin A or B were performed on diarrheal stool specimens collected from patients in 2015 and 2016. Specimens were also cultured and isolates of C. difficile characterized by PCR ribotyping and detection of toxin genes. In total, 437 specimens were collected and fecal toxin was detected in 3.0%. A further 16.2% of specimens were GDH positive and toxin negative. After culture, toxigenic strains were isolated from 10.3% and nontoxigenic strains from 12.4% of specimens. The most prevalent PCR ribotypes (RTs) were RT 017 (20.0%) and RT 043 (10.0%). The high prevalence of RT 017 and nontoxigenic strains in Malaysia and in neighboring Thailand and Indonesia suggests that they localize to the region of Southeast Asia, with an implication that they may mediate the burden of CDI in the region.

Details

Title: High prevalence of toxigenic and nontoxigenic clostridium difficile strains in Malaysia
Authors/Creators: T.V. Riley (Author/Creator) - The University of Western Australia
D.A. Collins (Author/Creator) - The University of Western Australia
R. Karunakaran (Author/Creator) - University of Malaya
M.A. Kahar (Author/Creator)
A. Adnan (Author/Creator) - University of Malaya
S.A. Hassan (Author/Creator) - Universiti Sains Malaysia
N.H. Zainul (Author/Creator) - Universiti Sains Malaysia
F.R.M. Rustam (Author/Creator) - Universiti Teknologi MARA
Z.A. Wahab (Author/Creator) - Hospital Sungai Buloh
R. Ramli (Author/Creator) - National University of Malaysia
Y.Y. Lee (Author/Creator) - Universiti Sains Malaysia
H. Hassan (Author/Creator) - University of Malaya
A.B. Onderdonk (Author/Creator)
Publication Details: Journal of Clinical Microbiology, Vol.56(6)
Publisher: American Society for Microbiology
Identifiers: 991005543818207891
Copyright: © 2018 American Society for Microbiology
Murdoch Affiliation: School of Veterinary and Life Sciences
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 1 Clinical & Life Sciences; 1.120 Inflammatory Bowel Diseases & Infections; 1.120.1133 Clostridium Infections
Web Of Science research areas: Microbiology
ESI research areas: Microbiology