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High-resolution HLA-DQB1 typing using the polymerase chain reaction and sequence-specific primers
Journal article   Peer reviewed

High-resolution HLA-DQB1 typing using the polymerase chain reaction and sequence-specific primers

C.G. Mullighan, M. Bunce and K.I. Welsh
Tissue Antigens, Vol.50(6), pp.688-692
1997
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Abstract

Polymorphism at HLA-DQB1 is known to influence tissue compatibility and disease susceptibility; however, current DQB1 typing methods are unable to distinguish the 32 currently recognized DQB1 alleles. We have developed a 32-reaction PCR-SSP method capable of differentiating all DQB1 alleles that differ in amino acid sequence. This method can resolve all heterozygous combinations of DQB1 alleles, with the exception of several combinations involving alleles not thus far detected in Caucasoid populations.

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Source: InCites

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Citation topics
1 Clinical & Life Sciences
1.6 Immunology
1.6.607 MHC Diversity
Web Of Science research areas
Cell Biology
Immunology
Pathology
ESI research areas
Molecular Biology & Genetics
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